Description
Nt.BstNBI is a nicking endonuclease that cleaves only one strand of double-stranded DNA (dsDNA) substrates, generating nicks without fully cutting the dsDNA.
Specifications
|
Cat NO. |
15217ES84 |
|
Specification |
2000 U |
|
Recognition Site |
5'-GAGTCNNNN↓N-3' |
|
Enzyme Activity |
10 U/μL |
|
Reaction Conditions |
1× Nt.BstNBI Buffer; incubation at 55°C |
|
Inactivation Conditions |
Incubation at 80°C for 20 min |
|
Unit Definition |
One unit (U) is defined as the amount of enzyme required to completely convert 1 µg of supercoiled pUC19 DNA into open circular form in a 50 μL reaction volume within 1 hour at 55°C. |
Components
|
Components No. |
Name |
15217ES84(2000 U) |
|
15217-A |
Nt.BstNBI (10 U/μL) |
200 μL |
|
15217-B |
10×Nt.BstNBI Buffer |
2×1 mL |
Shipping and Storage
This product should be stored at -25℃~-15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
Inquiry
You may also like
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.