Description
The FuniCut™ series of restriction enzymes are engineered via recombinant DNA technology to enable precise DNA digestion within 5–15 minutes. These fast-acting restriction enzymes are suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and other substrates. All enzymes in the FuniCut™ series share a universal digestion buffer, simplifying reaction setup. Additionally, they exhibit high activity redundancy, enabling efficient digestion even with excess substrate or challenging templates.
Specifications
|
Enzyme |
Basl |
|
Recognition Site |
5'-GGTCTC(N)₁↓-3' 3'-CCAGAG(N)₅↑-5' |
|
Recommended Reaction Conditions |
1× FuniCut™ Buffer; incubate at 37°C. |
|
Enzyme Activity |
20 U/μL |
|
Inactivation Conditions |
Incubate at 80°C for 20 minutes. |
|
Isolytic enzymes |
PalAI and SgsI |
|
Methylation modification affects |
Dam, Dcm, EcoKI, and EcoBI: No effect; CpG: Complete sequence overlap, cleavage blocked. |
Components
|
Components No. |
Name |
15005ES50 |
|
15005-A |
FuniCut™ BsaI |
50 μL |
|
15005-B |
10×FuniCut™ Buffer |
1 mL |
|
15005-C |
10×FuniCut™ Color Buffer |
1 mL |
[Note]:*10xFuniCut™ Color Buffer includes red and yellow tracking dyes, allowing direct use of the product for gel electrophoresis. The red dye migrates similarly to a 2500 bp double-stranded DNA fragment in 1% agarose gel; the yellow dye migrates similarly to a 10 bp double-stranded DNA fragment in 1% agarose gel.
Shipping and Storage
This product should be stored at -25℃~-15℃ for up to 2 years.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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