Description
FuniCut™ enzymes are a series of engineered restriction enzymes that are capable of fast DNA digestion. All FuniCut™ enzymes show superior activity in the universal FuniCut™ Buffer and are able to digest DNA in 5~15 minutes. This enables any combination of restriction enzymes to work simultaneously in one reaction tube and eliminates the need for sequential digestions. FuniCut™ enzymes have passed multiple strict quality control steps, and can be used to digest plasmid, genomic and viral DNA as well as PCR products.
Features
- Rapid digestion: DNA was digested rapidly by enzyme for 5-15 min
- Universal buffer: one tube reaction, simplifying the process
- Stringent quality control
Applications
- Molecular cloning
- Southern blotting
- SNP
- Southern blotting
- Genotyping
Specifications
| Enzyme | DpnI |
| Cutting Site | 5'-GAm6↓TC-3' 3'-CT↑Am6G-5' |
| Optimal Reaction Temperature | 37℃ |
| Sensitive to Heat Inactivation | YES |
| Type IIs RE | NO |
| Methylation Sensitivity | Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not EcoKI Methylation-Sensitive, EcoBI Methylation-Sensitive |
| Star Activity | 3 h incubation did not show star activity, and delayed enzyme digestion might show star activity |
| Isoschizomers | NO |
Components
| Components No. | Name | 15052ES50 (50 T) |
| 15052-A | FuniCut™ DpnI | 50 μL |
| 15052-B | 10× FuniCut™ Buffer | 1 mL |
| 15052-C | 10× FuniCut™ Color Buffer | 1 mL |
Storage
The product should be stored at -25℃ ~ -15℃ for two years. Please avoid repeated freeze-thaw.
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