Description
This product is a type II restriction endonuclease, a recombinant protein encoded by the BspQI gene from Bacillus sphaericus expressed in E. coli. It recognizes the sequence 5'-GCTCTTCN1/N4-3' and is mainly used for digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends, obtaining specific sticky ends.
Applications
Digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends.
Digesting DNA to obtain specific sticky ends.
Specifications
|
Recognition site |
5'-GCTCTTC(N)↓-3' 3'-CGAGAAG(N)4↑-5' |
|
Concentration |
10 U/µL |
|
Definition of Unit |
The amount of enzyme required to digest 1 µg of λDNA in a 50 µL reaction volume at 50°C within 1 hour is defined as one unit (U) of activity. |
|
Reaction conditions |
React at 50℃ for 60 min |
Components
|
Name |
15202ES76 |
15202ES86 |
15202ES92 |
|
BspQI (10 U/μL) |
50 μL |
250 μL |
1 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 1 year.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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