TRIeasy™ Total RNA Extraction Reagent is a total RNA extraction reagent suitable for various animal and plant tissues, as well as bacterial tissues and cells. It has a strong lysis capability, allowing for the rapid lysis of cell and tissue samples while effectively inhibiting RNA degradation, thus maintaining RNA integrity. Samples can be fully lysed in this reagent, followed by the addition of chloroform and centrifugation to form a supernatant layer, interphase, and organic layer (bright red lower layer). RNA is distributed in the upper aqueous phase, and after collecting the supernatant, total RNA can be obtained through isopropanol precipitation. The extracted total RNA has high purity, with minimal protein and genomic DNA contamination, and can be directly used in various molecular biology experiments such as Northern blotting, dot hybridization, mRNA purification, in vitro translation, RT-PCR, poly(A)+ selection, RNase protection analysis, and constructing cDNA libraries.

In addition, DNA and proteins in the sample can also be recovered through precipitation. Ethanol precipitation can extract DNA from the interphase, while adding isopropanol to the organic phase can precipitate proteins. This product is simple and quick to operate, with all procedures completed within one hour. It has good lysis efficiency for small amounts of tissue (50-100 mg) and cells (5×10 ⁶ ), as well as larger amounts of tissue (≥1 g) and cells (>10 ⁷ ).

Features

Permits the isolation of RNA, DNA, and protein from the same sample

Offers superior lysis capability, even with difficult sample types
Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria

Applications

a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.

Specifications

Shipping and Storage

This product should be stored at 2~8℃ for 1 year.

Figures

Documents:

Safety Data Sheet

10606ES-MSDS-HB240223.PDF

Manuals

10606_Manual_Ver. EN20250205_EN.pdf

Citations & References:

[1] Wang Y, Wang Y, Song D, et al. An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination. Nat Chem. 2022;14(3):350-359. doi:10.1038/s41557-021-00847-3(IF:24.427)

[2] Niu B, Liu J, Lv B, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12(1):2809. Published 2021 May 14. doi:10.1038/s41467-021-23048-5(IF:14.919)

[3] Shui S, Zhao Z, Wang H, Conrad M, Liu G. Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway. Redox Biol. 2021;45:102056. doi:10.1016/j.redox.2021.102056(IF:11.799)

[4] Fang Y, He Y, Wu C, et al. Magnetism-mediated targeting hyperthermia-immunotherapy in "cold" tumor with CSF1R inhibitor. Theranostics. 2021;11(14):6860-6872. Published 2021 May 3. doi:10.7150/thno.57511(IF:11.556)

[5] Jiang L, Yin X, Chen YH, et al. Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. Theranostics. 2021;11(4):1703-1720. Published 2021 Jan 1. doi:10.7150/thno.43895(IF:11.556)

[6] Tang X, Deng Z, Ding P, et al. A novel protein encoded by circHNRNPU promotes multiple myeloma progression by regulating the bone marrow microenvironment and alternative splicing. J Exp Clin Cancer Res. 2022;41(1):85. Published 2022 Mar 8. doi:10.1186/s13046-022-02276-7(IF:11.161)

[7] Zhou W, Li X, Wang Y, et al. Physiological and transcriptomic changes of zebrafish (Danio rerio) embryos-larvae in response to 2-MIB exposure. J Hazard Mater. 2021;416:126142. doi:10.1016/j.jhazmat.2021.126142(IF:10.588)

[8] Mo X, Du S, Chen X, et al. Lactate Induces Production of the tRNAHis Half to Promote B-lymphoblastic Cell Proliferation. Mol Ther. 2020;28(11):2442-2457. doi:10.1016/j.ymthe.2020.09.010(IF:8.986)

[9] Hao W, Han J, Chu Y, et al. Lower fluidity of supported lipid bilayers promotes neuronal differentiation of neural stem cells by enhancing focal adhesion formation. Biomaterials. 2018;161:106-116. doi:10.1016/j.biomaterials.2018.01.034(IF:8.806)

[10] Zou G, Zhang X, Wang L, et al. Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. Theranostics. 2020;10(15):6839-6853. Published 2020 May 22. doi:10.7150/thno.43622(IF:8.579)

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