Description
Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. The core component Hieff Unicon™ Taq DNA polymerase uses antibody hot start to effectively inhibit non-specific amplification due to primer annealing during sample preparation. At the same time, the formula adds the promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents (30 ~ 70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to most qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.
Features
- Built-in visual indicator: It contains an inert blue dye that does not interfere with real-time PCR and provides higher visibility in tubes or Wells.
- High specificity: It contains Taq DNA polymerase and is tightly controlled by an antibody-mediated heat-priming mechanism, thereby avoiding nonspecific amplification.
- Wide instrument compatibility: It is compatible with most qPCR instruments and eliminates the need to adjust the concentration of ROX on different instruments
Applications
- Gene Expression
Specifications
Concentration | 2× |
Detection method | SYBR Green dye |
PCR method | qPCR |
Polymerase | Taq DNA polymerase |
Type of sample | DNA |
Application equipment | Most qPCR instruments |
Product Type | Premix for real-time fluorescence quantitative PCR |
Apply to (application) | Gene Expression |
Components
Components No. | Name | 11184ES03 | 11184ES08 | 11184ES50 | 11184ES60 |
11184 |
Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) |
1 mL | 5×1 mL | 50×1 mL | 100×1 mL |
Shipping and Storage
The product is shipped with ice packs and can be stored at -15℃ ~ -25℃ for 18 months. The product contains fluorescent dyes, so it is necessary to avoid strong light irradiation when storing or preparing the reaction system.
Figures
Figure 1. High sensitivity: the ability to detect a single copy.
Figure2. High resolution & Excellent reproducibility of duplicate wells.
Figure3. Excellent detection rate and specificity: broadly suited for the amplification of 30-70% GC.
Cited from "VCP enhances autophagy-related osteosarcoma progression by recruiting USP2 to inhibit ubiquitination and degradation of FASN, Cell Death & Disease, volume 15, Article number: 788 (2024) "
Cited from "A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens. Cell . 2020 Sep 3;182(5):1109-1124.e25. doi: 10.1016/j.cell.2020.07.020. "
[1] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[2] Wang S, Li Y, Zhong L, et al. Efficient gene editing through an intronic selection marker in cells. Cell Mol Life Sci. 2022;79(2):111. Published 2022 Jan 31. doi:10.1007/s00018-022-04152-1(IF:9.261)
[3] An LL, Zhao X, Gong XY, et al. Promoter Binding and Nuclear Retention Features of Zebrafish IRF Family Members in IFN Response. Front Immunol. 2022;13:861262. Published 2022 Apr 6. doi:10.3389/fimmu.2022.861262(IF:7.561)
[4] Zhao Y, Wang HP, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions. Bioresour Technol. 2021;319:124150. doi:10.1016/j.biortech.2020.124150(IF:7.539)
[5] Shu C, Wang L, Zou C, et al. Function of Foxl2 and Dmrt1 proteins during gonadal differentiation in the olive flounder Paralichthys olivaceus [published online ahead of print, 2022 Jun 16]. Int J Biol Macromol. 2022;215:141-154. doi:10.1016/j.ijbiomac.2022.06.098(IF:6.953)
[6] Wang S, Huang J, Liu F, et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021;14:7107-7130. Published 2021 Dec 20. doi:10.2147/JIR.S344990(IF:6.922)
[7] Wang J, Hu R, Wang Z, et al. Establishment of Immortalized Yak Ruminal Epithelial Cell Lines by Lentivirus-Mediated SV40T and hTERT Gene Transduction. Oxid Med Cell Longev. 2022;2022:8128028. Published 2022 Mar 25. doi:10.1155/2022/8128028(IF:6.543)
[8] Liu W, Guan Y, Qiao S, et al. Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin-Induced Premature Aging in Adult Mice. Oxid Med Cell Longev. 2021;2021:9942090. Published 2021 Aug 6. doi:10.1155/2021/9942090(IF:6.543)
[9] Qian Z, Liu C, Li H, et al. Osteocalcin Alleviates Lipopolysaccharide-Induced Acute Inflammation via Activation of GPR37 in Macrophages. Biomedicines. 2022;10(5):1006. Published 2022 Apr 27. doi:10.3390/biomedicines10051006(IF:6.081)
[10] Zhao X, Huang Y, Li X, et al. Full integration of nucleic acid extraction and detection into a centrifugal microfluidic chip employing chitosan-modified microspheres [published online ahead of print, 2022 Jun 27]. Talanta. 2022;250:123711. doi:10.1016/j.talanta.2022.123711(IF:6.057)
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