2× Hieff™ PCR Master Mix (With Dye) _ 10102ES
2× Hieff™ PCR Master Mix (With Dye) _ 10102ES
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2× Hieff™ PCR Master Mix (With Dye) _ 10102ES2× Hieff™ PCR Master Mix (With Dye) _ 10102ES
2× Hieff™ PCR Master Mix (With Dye) _ 10102ES
2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×Hieff™ PCR Master Mix simplifies PCR procedure and reduces contamination. Features Convenient, ready-to-use...

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2× Hieff™ PCR Master Mix (With Dye) _ 10102ES

YeasenSKU: 10102ES03
Size: 1mL 100T
Price:
$15.00
Stock:
In stock

2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×Hieff™ PCR Master Mix simplifies PCR procedure and reduces contamination.

Features

Convenient, ready-to-use mix

Thermostable—half-life is more than 40 min at 95°C

Generates PCR products with 3'-dA overhangs

Applications

Genetyping

Routine PCR amplification of DNA fragments

High throughput PCR

DNA labeling

Specifications

Fidelity (vs. Taq) 1 ×
Hot Start No
Overhang 3'-A
Polymerase Taq DNA Polymerase
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Product Type PCR Master Mix (2×)

Components

Components No. Name 10102ES03 10102ES08 10102ES50 10102ES60
10102 2×Hieff™ PCR Master Mix (With Dye) 1 mL 5×1 mL 50×1 mL 100×1 mL

Figures

Figure1. The expected 1.2 kb PCR products can be amplified with 2×Hieff PCR Master Mix.

The Master Mix was stored at -20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.

Storage

The 2×Hieff™ PCR Master Mix products should be stored at -15℃ ~ -25℃ for 2 years.

FAQ

Both 10102ES and 10103ES belong to the 2× Hieff® PCR Master Mix series, with only one core difference: whether they contain bromophenol blue dye. 10102ES can be directly used for gel electrophoresis afterward. In contrast, 10103ES does not contain the bromophenol blue indicator, so DNA loading buffer must be added during electrophoresis.

Citations

Documents

Manual
10102ES-Manual-Ver.EN20250904.pdf
MSDS
10102_MSDS_HB220429_EN_1652931562593.PDF

Applications

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Inquiry

FAQ

The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.

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