Description
Agarose is a purified linear galactoglycan hydrophilic colloid derived from agar or agar-containing algae. It is a linear polymer composed of β-d-galactosyl (1-4) linked to 3, 6-dehydrated-L-galactosyl. As a gel reagent, it is commonly used for routine nucleic acid analysis by gel electrophoresis or blotting (such as Northern or Southern) and for protein applications, such as radiative immunodiffusion (RID) assay.
Applications
Gel electrophoresis
Components
|
Name |
10208ES08 |
10208ES60 |
10208ES70 |
|
Agarose |
5 g |
100 g |
500 g |
Specifications
|
9012-36-6 |
|
|
Appearance |
White to off-white powder |
|
Gel Strength (1.0%) |
≥1200 (g/cm2) |
|
Gel Point (1.5%) |
36±1.5℃ |
|
Melting Point (1.5%) |
88±1.5℃ |
|
EEO (-mr) |
≤0.13 |
|
Sulfate (%) |
≤0.15% |
|
Moisture |
≤10% |
|
DNase |
None Detected |
|
RNase |
None Detected |
|
Protease |
None Detected |
Storage
The Agarose products should be stored at room temperature for 5 years.
Cited from“Gain-of-Function Mutation of Card14 Leads to Spontaneous Psoriasis-like Skin Inflammation through Enhanced Keratinocyte Response to IL-17A. Immunity. 2018;49(1):66-79.e5. doi:10.1016/j.immuni.2018.05.012”
FAQ
Q: Why do the bands that are produced spread out?
A: The gelation time might be too short, resulting in uneven gelation. The minimum gelation time for 1% agarose is 30 minutes. Q2: How large of a band can the high-resolution agarose separate?
A: The resolution of high-resolution agarose and 3:1 agarose is both approximately 2% (that is, for a fragment of 30bp, it can separate 30*0.02; for a 100bp fragment, it is 100*0.02.)
Q: Can low-melting-point agarose be used for pulsed-field gel electrophoresis?
A: No. The requirements for pulsed field gel electrophoresis are that the electroosmosis should be very low and the intensity should be very high.
Q: Is low-melting-point agarose sterile and free of nucleases? What about other types of agarose?
A: This type of agarose is sterile and free of nucleases, and has a low melting point. It is commonly used for cell plaque assays. It can be safely used for cell culture. Other types of agarose are also sterile and free of nucleases.
Q: Why is agarose relatively soft?
A: The hardness and gel strength are related to the gel. The agarose is derived from agar. There will be certain differences in strength among different batches. It's impossible for all batches to be exactly the same. The strength index of agarose is greater than or equal to 1200. As long as it is above this range, it is considered qualified.
Documents:
Citations & References:
[1] Wang M, Zhang S, Zheng G, et al. Gain-of-Function Mutation of Card14 Leads to Spontaneous Psoriasis-like Skin Inflammation through Enhanced Keratinocyte Response to IL-17A. Immunity. 2018;49(1):66-79.e5. doi:10.1016/j.immuni.2018.05.012(IF:19.734)
[2] Zhang Y, Ding H, Wang X, et al. MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal. Cell Rep. 2021;37(5):109949. doi:10.1016/j.celrep.2021.109949(IF:9.423)
[3] Zhu Z, Zhang L, Sheng R, Chen J. Microfluidic-Based Cationic Cholesterol Lipid siRNA Delivery Nanosystem: Highly Efficient In Vitro Gene Silencing and the Intracellular Behavior. Int J Mol Sci. 2022;23(7):3999. Published 2022 Apr 3. doi:10.3390/ijms23073999(IF:5.924)
[4] Zhao C, Yang D, Ye Y, et al. Inhibition of Pim-2 kinase by LT-171-861 promotes DNA damage and exhibits enhanced lethal effects with PARP inhibitor in multiple myeloma. Biochem Pharmacol. 2021;190:114648. doi:10.1016/j.bcp.2021.114648(IF:5.858)
[5] Yu J, Yang W, Xing S, et al. Blended gold/MnO<sub>2</sub>@BSA nanoparticles for fluorometric and magnetic resonance determination of ascorbic acid. Mikrochim Acta. 2019;186(2):89. Published 2019 Jan 10. doi:10.1007/s00604-018-3205-8(IF:5.479)
[6] Zheng X, Xu W, Sun R, Yin H, Dong C, Zeng H. Synergism between thioredoxin reductase inhibitor ethaselen and sodium selenite in inhibiting proliferation and inducing death of human non-small cell lung cancer cells. Chem Biol Interact. 2017;275:74-85. doi:10.1016/j.cbi.2017.07.020(IF:5.194)
[7] Zhou J, Xiong R, Zhou J, et al. Involvement of m6A regulatory factor IGF2BP1 in malignant transformation of human bronchial epithelial Beas-2B cells induced by tobacco carcinogen NNK. Toxicol Appl Pharmacol. 2022;436:115849. doi:10.1016/j.taap.2021.115849(IF:4.219)
[8] Zhou Y, Liu J, Cai S, Liu D, Jiang R, Wang Y. Protective effects of ginsenoside Rg1 on aging Sca-1⁺ hematopoietic cells. Mol Med Rep. 2015;12(3):3621-3628. doi:10.3892/mmr.2015.3884(IF:2.952)
[9] Zheng SY, Ma LL, Wang XL, et al. RPA-Cas12aDS: A visual and fast molecular diagnostics platform based on RPA-CRISPR-Cas12a method for infectious bursal disease virus detection. J Virol Methods. 2022;304:114523. doi:10.1016/j.jviromet.2022.114523(IF:2.014)
[10] Sun Z, Zhang H, Xie K, Tan X, Zhang H, Chen J. Rice Black-streaked Dwarf Virus Preparation and Infection on Rice. Bio Protoc. 2017;7(24):e2651. Published 2017 Dec 20. doi:10.21769/BioProtoc.2651(IF:0.000)
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