Description
2×Hieff™ Ultra-Rapid II HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency. It is very suitable for PCR amplification of most colonies such as E. coli, Agrobacterium, and yeast. The extension speed of amplification of complex templates within 3 kb can reach 1 sec/kb, 3-6 kb to 3 sec/kb, 6-10 kb to 5 sec/kb and 10 kb to 10 sec/kb; the amplification speed of simple templates such as plasmid within 6 kb can reach 1 sec/kb, which can greatly save PCR reaction time. Meanwhile, Mix contains dNTPs, Mg2+, and it can be amplified only with adding primers and template. In addition, Mix contains red tracer dye, which can be used by electrophoresis directly after the end of the reaction. The protective agent added to the system enables the product to maintain a stable activity after repeated freezing and thawing. The 3′ end of the PCR product bands A and can be easily cloned into the T vector.
Features
Faster – Bacterial plasmids up to 3 kb can be processed in as little as 1 second per kb.
More Versatile – Efficiently handles GC content up to 70% and fragment lengths up to 10 kb.
More Reliable – Validated on hundreds of fragments across over ten species.
Higher Efficiency – High specificity with detection rates up to 100%.
More Stable – Maintains stability after 100 freeze–thaw cycles, with a shelf life of up to two years.
Applications
Crispr PCR
Colony Direct PCR
Genotyping
Specifications
|
Product specification |
Master Mix |
|
Concentration |
2× |
|
Hot Start |
Built-in Hot Start |
|
Overhang |
3 '-A |
|
Reaction speed |
Rapid |
|
Size (Final Product) |
Up to 15 kb |
|
Conditions for transportation |
Dry ice |
Figures
1. Crispr Genotyping
Cited from: Viruses 2024, 16(9), 1382;
2. Nest PCR
Cited from: Front. Microbiol. 15, 2024
3. Colony Direct PCR
Fig 1. Fast Colony Direct PCR with E. coli and Agrobacterium samples using 4 different speed of 1: 1s/kb, 2: 3s/kb, 3: 5s/kb and 4: 10s/kb. M: 10,000 DNA Marker (10505ES).
4. Long PCR with Genomic DNA
Fig. 2. Fast amplification of 1-10 kb mouse gDNA shows high yield and a high speed at 10 sec/kb. M: 10,000 DNA Marker (10505ES).
5. High Stability 
Storage
The products should be stored at -25~-15℃ for 2 years.
Documents:
10167ES 2×Hieff® Ultra-Rapid II HotStart PCR Master Mix-Ver.EN20240517.pdf
Related Blog
YEASEN Rapid PCR Mix: The 3 kb colony PCR is fast to 1s / kb! Detection rate is up to 100%!
PCR, Nucleic Acid Electrophoresis, Laboratory Techniques Handbook
Free Guide: Top 5 PCR Optimization Tips
Citations
[1] Rapid, sensitive, and visual detection of mandarin fish ranavirus and infectious spleen and kidney necrosis virus using an RPA-CRISPR/Cas12a system，Front. Microbiol. , 13 December 2024, Sec. Virology, Volume 15, 2024
[2] CRISPR/Cas9-Mediated Resistance to Wheat Dwarf Virus in Hexaploid Wheat (Triticum aestivum L.) Viruses 2024, 16(9), 1382;
[3] The adhesion-GPCR ADGRF5 fuels breast cancer progression by suppressing the MMP8-mediated antitumorigenic effects. Cell Death Dis 15, 455 (2024).
[4] Zhong, X., Yang, Y., He, H. et al. Integrating multi-cohort machine learning and clinical sample validation to explore peripheral blood mRNA diagnostic biomarkers for prostate cancer. Cancer Cell Int 25, 158 (2025)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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