Beschrijving
This Kit is designed for rapid and efficient RNA extraction from Gram-positive and Gram-negative bacteria.Utilizing a proprietary silica- membrane column technology combined with optimized buffers, this kit effectively removes contaminants such as proteins, metabolites, and genomic DNA. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning.
Specifications
|
Cat.No. |
19301ES50 |
|
Size |
50 T |
Components
|
Category |
Components No. |
Name |
19301ES50 |
|
Part I |
19301-A |
Lysozyme |
20 mg |
|
Part II |
19301-B |
DNA-Removing Column B4 |
50 units |
|
19301-C |
Hieff™ RNA Column B4 |
50 units |
|
|
19301-D |
2 mL Collection Tube B4 |
100 units |
|
|
19301-E |
Lysis Buffer(LB Buffer B4) |
25 mL |
|
|
19301-F |
PL Buffer B4 |
40 mL |
|
|
19301-G |
Wash Buffer B4* |
13 mL(add 52 mL ethanol before use) |
|
|
19301-H |
TE Buffer pH 8.0 |
6 mL |
|
|
19301-I |
RNase-free H2O |
5 mL |
Features
Ø Wide applicability: can be used for bacterial cultures such as Escherichia coli, Pseudomonas aeruginosa, and Gram-positive bacteria.
Ø Quick and easy: the operation can be completed within 40 minutes.
Ø High RNA purity: DNase I on-column digestion effectively removes gDNA.
Ø Safe and environmentally friendly: no need to use organic matter such as phenol and chloroform for extraction.
Applications
Bacterial RNA extraction；RNA extraction from Gram-positive bacteria；Gram-negative bacteria, etc.
Shipping and Storage
Part I components should be stored at 4~8℃ for one year.
Part II components should be stored at room temperature for one year.
Figures
|
Suppliers |
Concentration |
OD260/OD280 |
OD260/OD230 |
|
Yeasen-19031ES |
159.40/193.18 |
1.895/1.925 |
2.177/2.101 |
|
Supplier T* |
182.01/179.31 |
1.855/1.922 |
0.728/1.206 |
Table 1. E. coli extract product concentration
Figure 1. Electrophoresis of E. coli RNA extraction products
Take 4 portions of 2 mL of E. coli suspension, use 19301 and similar products from supplier T brand to extract total RNA, and elute with 50 ul RNase-free H2O. Then take 1 ul of the eluate to determine the concentration. Take 5 ul of the eluate to identify the RNA integrity and band brightness. The results show that the 19301 extraction product has a high yield and good RNA integrity.
Documents:
Safety Data Sheet
19301_Manual_Ver. EN20250514_EN.pdf
Citations & References:
[1] Ma X, Hou M, Liu C, Li J, Ba Q, Wang H. Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans. J Hazard Mater. 2022;421:126723. doi:10.1016/j.jhazmat.2021.126723(IF:10.588)
[2] Wang Y, Hu W, Deng Z, He X. Rapid identification of magnesium ascorbyl phosphate utilizing phosphatase through a chromogenic change-coupled activity assay. Appl Microbiol Biotechnol. 2021;105(7):2901-2909. doi:10.1007/s00253-021-11229-7(IF:4.813)
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FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
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