Beschrijving
The SuperMethyl™-Fast Bisulfite Conversion Kit from Ellis Bio offers the revolutionary fastest and most efficient bisulfite conversion solution available for DNA methylation analysis. Featuring a newly engineered, ready-to-use fast bisulfite conversion reagent and a magnetic bead-based DNA purification method, this kit streamlines the workflow by combining conversion and purification in one seamless process. Simply add the SuperMethyl™-Fast Bisulfite Conversion Reagent to your sample and incubate for 6 - 8 minutes at 98°C (standard protocol is 7 minutes).
Complete bisulfite conversion and DNA purification in under 1 hour, this kit not only delivers speed but also precision, achieving over 99.5% conversion efficiency while minimizing DNA degradation. Its versatility makes it indispensable for both research and clinical applications.
With an unmatched rapid workflow and exceptional performance, the SuperMethyl™-Fast Bisulfite Conversion Kit ensures reliable and reproducible results, making it the go-to choice for researchers who require both precision and quick turnaround times.
Features
- Fastest Conversion Available:10X faster than leading industry kits
- Exceptional Performance:Excellent methylation protection; Compatible with a wide range of DNA inputs.
- Versatile Applications:Optimized for PCR, microarrays, endonuclease digestion, and next-generation sequencing (NGS).
- Cost-Effective:At just $179.99, competitively priced with a price-match guarantee.
Components
|
Component |
Volume and Ǫuantity |
|
SuperMethyl - Fast Conversion Reagent |
1.5 mL x 7 vials |
|
SuperMethyl - Fast Binding Buffer |
28 mL |
|
SuperMethyl - Fast Wash Buffer* |
14 mL* |
|
SuperMethyl - Fast Desulphonation Buffer |
11 mL |
|
SuperMethyl - Fast Elution Buffer |
1.8 mL |
|
SuperMethyl - Fast Purification Beads |
0.55 mL |
* SuperMethyl - Fast Wash Buffer requires the addition of 5C mL 100% ethanol (EtOH) before first use.
Shipping Conditions
No Cold Chain Required; Shipped at Room Temperature
Precautions / Handling Instructions:
Upon receipt of the kit, store the Conversion Reagent at 4°C.
Figures
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2.
mESC gDNA with 1% unmethylated lambda DNA spike-in were converted with SuperMethylTM Fast and other suppliers’ bisulfite conversion kits. False positive rates were calculated post-NGS as the percentage of CpG and non-CpG cytosines showing >1% unconverted C.
Documents:
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