The Hieff NGS™ MagSP rRNA Removal Kit (Bacteria, G⁻ and G⁺) with Purification Beads uses streptavidin-labeled magnetic beads to deplete ribosomal RNA from bacterial (Gram-negative and Gram-positive) total RNA, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. The kit delivers efficient rRNA removal from both intact and partially degraded total RNA. The rRNA-depleted RNA is suitable for downstream applications including high-throughput sequencing of mRNA and non-coding RNA—which markedly increases the proportion of informative reads—as well as cDNA synthesis and other downstream uses.
Product Information
|
Product Name |
Catalog No. |
Size |
|
Hieff NGS™ MagSP rRNA Removal Kit (Bacteria(G⁻ and G⁺)) with purification beads V2 |
12264ES04 |
4 T |
|
12264ES24 |
24 T |
|
|
12264ES96 |
96 T |
Component Information
|
Component |
Code |
Name |
12264ES04 |
12264ES24 |
12264ES96 |
|
BOX I |
12264-A |
PM, Probe Mix |
13.5 μL |
80 μL |
317 μL |
|
12264-B |
HB, Hybridization buffer |
25 μL |
150 μL |
600 μL |
|
|
12264-C |
DB, Depletion buffer |
800 μL |
4.6 mL |
18.5 mL |
|
|
BOX II |
12264-D |
SMB, Streptavidin-coated magnetic beads |
176 μL |
1056 μL |
4.23 mL |
|
12264-E |
PB, Purification beads |
795 μL |
4.75 mL |
19 mL |
Features
Pan-bacterial ribosomal RNA removal kit (universal across bacterial species).
Does not include archaeal depletion.
Magnetic-bead workflow with broad species probe coverage.
Applications
Microbial transcriptomics.
Meta-transcriptomics.
Storage
FAQ
Citations
Documents
Applications
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