This product is designed for the isolation of total RNA from animal tissues (e.g., liver, kidney, spleen, etc.). It utilizes uniquely engineered magnetic beads and an optimized buffer system to efficiently capture released nucleic acids. The extracted RNA is of high purity, stable, and reliable in quality, making it suitable for downstream applications such as RT-PCR, Northern blotting, and in vitro translation. When used with automated nucleic acid extraction instruments, this kit enables high-throughput RNA purification.

Features

• Wide Sample Coverage: Compatible with various animal tissues and aquatic samples.
• Rapid, Automated Workflow: Complete extraction in <1 hour; fully compatible with Yeasen 16/48/96-channel extractors.
• Pure, DNA-Free RNA: Built-in DNase I ensures clean RNA for RT-PCR and NGS applications.

Components

[Note]:

For 18605ES50, Add 20 mL of absolute ethanol to Protein Removal Buffer PE* (18605-B), and 100 mL of absolute ethanol to the Wash Buffer RW(18605-C).

For 18605ES60, Add 40 mL of absolute ethanol to Protein Removal Buffer PE* (18605-B), and 200 mL of absolute ethanol to the Wash Buffer RW(18605-C).

Storage

This product should be stored at room temperature for 2 years.

Application

Tissue RNA Extraction; High-purity RNA Extraction; Gene Expression Analysis.

Figures

 High Yield and High Integrity RNA Extraction

Figure 1. Comparison of RNA Extraction from Diverse Samples

RNA was extracted from 10 mg mouse liver, 10 mg mouse spleen, and 4×10⁶⁶ 293T cells using Yeasen Kit (Cat. No. 18605) on an automated platform or a manual kit from Supplier O. Nanodrop quantification showed higher RNA yields with Yeasen. Agarose gel electrophoresis (3 µL RNA) revealed sharp 28S/18S rRNA bands and no genomic DNA contamination.

Cas Study-Verification of Magnetic Bead Aggregation During RNA Extraction

Background

During high-input RNA extraction workflows, magnetic bead aggregation was occasionally observed. It was hypothesized that excess nucleic acid content contributed to bead clumping. To address this issue, DNase I digestion and surfactant supplementation were evaluated.

Sample Input: Vero cells, ~1 × 10⁶ cells per sample

Kit used: Yeasen HieffTM Magnetic Tissue/Cell Total RNA Kit (18605ES)

Experimental Design

Results

These results demonstrate that DNase I digestion and surfactant supplementation do not negatively affect RNA yield, purity, or integrity, and can be considered effective strategies to mitigate magnetic bead aggregation when processing high cell-input samples.

Documents

Safety Data Sheet

18605_MSDS_Ver.EN20251120.pdf

Manuals

18605_Manual_Ver.EN20251120.pdf