Hieff NGS™ MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) 2.0 utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.

Features

Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.

Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).

High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.

Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.

Components

Applications

Gene Expression Research

Alternative splicing analysis

Detection and discovery of LncRNA(Non coding RNA)

dentification of selective polyadenylation sites

Fusion gene detection

Figures

1. Broad Compatibility Across Input Amounts and Sample Types

Figure 1. Superior rRNA Removal Efficiency Across a Broad Input Range

Library preparation was performed using the MaxUp™ rRNA Depletion Kit and a strand-specific RNA Library Preparation Kit on human, mouse, and rat total RNA samples. Unlike traditional kits limited to 1 µg input, MaxUp™ demonstrates consistent and efficient rRNA removal across a wide range of input amounts (100 ng to 1 µg), achieving <1% rRNA residual.

2. Effective rRNA Removal

Figure 2. Comparison of rRNA Removal Efficiency: Depletion vs. Enrichment

Using 1 μg of 293T total RNA, samples were processed using either oligo-dT enrichment or rRNA depletion methods. Sequencing analysis revealed that both methods achieved comparable levels of rRNA removal efficiency.

3. Optimized for FFPOptimized for FFPE & Degraded Samples

Figure 3. Robust rRNA Depletion Performance in FFPE Samples Across a Wide DV200 Range

MaxUp™ is optimized for challenging FFPE RNA, maintaining strong performance even with degraded transcripts. The kit demonstrates consistent and efficient rRNA removal across a broad DV200 range (20%–70%), achieving >98% depletion efficiency with a 100 ng input. This ensures high-quality, low-background library construction, making it highly compatible with low-quality samples (DV200 < 50%) for clinical and archival tissue research.

4. Automation-Ready Stability

Figure 4. Enhanced Stability and Automation Compatibility

MaxUp™ features a stabilized RNase H system that eliminates the need for fresh enzyme preparation, making it ideal for automated, high-throughput workflows. The kit maintains superior depletion efficiency (>95%) under various storage conditions, including extended periods at 37°C, RT, and 4°C.