Keterangan
Product Properties
|
Alternative Names |
CD8 alphaβ CD8A&CD8B |
|
Species |
Mouse |
|
Expression Sequence |
Lys28-Tyr196(CD8 alpha) acidic tail and Leu22-Thr175(CD8 beta) basic tail |
|
Accession |
P01731(CD8 alpha)&P10300(CD8 beta) |
|
Tag |
C-His-Flag |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 24.80 kDa (CD8 alpha) and 23.17 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-38 kDa (CD8 alpha) and 42-50 kDa (CD8 beta) based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
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FAQ
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