Keterangan
Product Properties
|
Alternative Names |
TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A, TNF superfamily member 15 |
|
Species |
Cynomolgus,Rhesus macaque |
|
Expression Sequence |
Leu72-Leu251 |
|
Accession |
A0A2K5UA22 |
|
Tag |
N-monomeric Fc |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 44.82 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE,> 90% as determined by HPLC |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Mouse DR3, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque TNFSF15, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. 【ELISA Data】 Immobilized Cynomolgus/Rhesus macaque TNFSF15, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
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