Keterangan
Product Propertie
|
Alternative Names |
Mesothelin,MPFSMRP,CAK1,MSLN |
|
Species |
Human |
|
Expression Sequence |
Ala488-Ser600 |
|
Accession |
Q13421-1 |
|
Tag |
C-hFc |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 39.30 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Human MSLN, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -85 to -65°C. The shelf life is one year after receipt of the product.After reconstitution, store under sterile conditions at -85 to -65°C. The shelf life is three months.
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FAQ
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