Keterangan
Product Properties
|
Alternative Names |
IL30,IL-27A,p28,Interleukin-27 |
|
Species |
Human |
|
Expression Sequence |
Arg21-Lys229(IL-27B)&Phe29-Pro243(IL-27A) |
|
Accession |
Q14213(IL-27B)&Q8NEV9(IL-27A) |
|
Tag |
C-His |
|
Expression System |
CHO |
|
Molecular Weight |
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
ELISA Data: Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA . |
|
Reconstitution Method |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃, valid for 3 months.
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FAQ
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