Keterangan
Product Properties
|
Property Name |
Property Details |
|
Alternative Names |
CD8A&CD8B (CD8 αβ heterodimer) |
|
Species |
Human |
|
Expression Sequence |
Ser22-Asp182 (CD8 α acidic tail) & Leu22-Pro170 (CD8 β basic tail) |
|
Accession |
P01732-1 (CD8 α) & P10966-1 (CD8 β) |
|
Tag |
C-His-Flag |
|
Expression System |
HEK293 |
|
Molecular Weight |
Predicted MW: 23.54 kDa (CD8 α), 22.62 kDa (CD8 β). Glycosylation shifts migration to 26-28 kDa (CD8 α) and 28-32 kDa (CD8 β) (SDS-PAGE). |
|
Purity |
>95% (SDS-PAGE & HPLC). |
|
Endotoxin |
<1.0 EU/μg (LAL method). |
|
Formulation |
Lyophilized from 0.22 μm filtered PBS (pH 7.4). 8% trehalose added as protectant. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Human CD8A&CD8B, His Tag at 0.5 μg/mL (100 μL/well). Dose response curve for Anti-CD8 Antibody (hFc Tag) shows EC₅₀ = 15.2 ng/mL. |
|
Reconstitution Method |
Centrifuge before opening. Reconstitute with distilled water to >100 μg/mL. Aliquot to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Lyophilized powder: Store at -25 to -15°C; stable for 1 year upon receipt.
Reconstituted protein: Store under sterile conditions at -85 to -65°C; stable for 3 months.
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