Keterangan
Product Properties
|
Synonyms |
Alkaline phosphatase, placental-like;PLAP-like;ALP-1;Alkaline Phosphatase |
|
Source |
Human Alkaline Phosphatase (Germ type) /ALPG Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile20-Asp503.[Accession | P10696] |
|
Molecular Weight |
The protein has a predicted MW of 79.33 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Human Alkaline Phosphatase (Germ Type) , hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl2 (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
1.The product should be stored at -25~-15℃ for 1 year from date of receipt.
2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
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