Keterangan
Product Properties
|
Synonyms |
ETGF; TGF-alpha; TGF type 1; TGFA |
|
Species |
Human |
|
Source |
HEK293 |
|
Sequence |
Val40-Ala89 |
|
Accession |
P01135-1 |
|
Tag |
N-hFc |
|
Molecular Weight |
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Bis-Tris PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA
SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
lyophilized powder |
Storage
Store at -25°C to -15°C. The product is valid for 1 year upon receipt.
After reconstitution, store at -85°C to -65°C and use within 3 months.
Reconstitution
Centrifuge before opening the vial. It is recommended to dissolve the lyophilized protein in distilled water. The reconstitution concentration should exceed 100 μg/ml.
Note
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
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Pertanyaan
FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
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