Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, hFc Tag

YeasenSKU: 94449ES25

Ukuran: 25 ug
Harga:
Harga penjualan$237.00

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Keterangan

Synonyms

beta3; Integrin alpha V beta 3;αVβ3; ITGAV&ITGB3

Source

Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail.[Accession | P06756-1(ITGAV)&P05106-1(ITGB3)]

Molecular Weight

The protein has a predicted MW of 131.90 kDa (ITGAV)&101.79 kDa (ITGB3). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAV)&105-130 kDa (ITGB3) based on SDS-PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Activity

ELISA Data: Immobilized Human ITGAV&ITGB3, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

Storage

The product should be stored at -25~-15℃ for 1 year from the date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Note

1.Please operate with lab coats and disposable gloves for your safety.

2.This product is for research use only.

 

 

 

 

 

 

 

 

 

 

 

 

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