Keterangan
Recombinant Protein A/G/L is a genetically engineered protein that combines all of the IgG binding spectra of Protein A, Protein G, and Protein L. It is a gene fusion product. It contains 5 IgG binding regions of Protein A, 2 regions of Protein G, and 5 binding regions of Protein L. The cell wall binding region, cell membrane binding region, and albumin binding region have been removed from recombinant Protein A/G/L to ensure maximum specific IgG binding. Recombinant Protein A/G/L is ideal for purifying polyclonal or monoclonal IgG antibodies. Protein A/G/L binds to IgG from humans, mice, rats, cattle, goats, sheep, rabbits, guinea pigs, pigs, dogs, and cats.
Specifications
|
Source |
E.coli |
|
Molecular Weight |
Approximately 89.3 kDa with a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 806 amino acids. |
|
Amino acid sequence |
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEE PRARPGSGSG KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAGC |
|
The concentration of 1 unit DO at 280 nm |
1.412 mg/ml |
|
Appearance |
Sterile filtered white lyophilized powder |
|
Purity |
> 95% by SDS-PAGE and HPLC analyses |
|
Endotoxin levels |
< 0.1 EU/μg of protein by the LAL method |
|
formula |
No additives, freeze-dried |
|
Reconstitution |
Distilled water or saline solution |
 Storage  Â
Transport with ice packs. Store at -20℃, valid for one year. It is recommended to store in small portions for first use to avoid repeated freezing and thawing.
Note
1.  For your safety and health, please wear a lab coat and disposable gloves when operating.
2. This product is for scientific research purposes only..
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FAQ
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