Recombinant Human FGF basic/FGF2/bFGF GMP Protein _ 91360ES
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Recombinant Human FGF basic/FGF2/bFGF GMP Protein _ 91360ES

Recombinant Human FGF basic/FGF2/bFGF GMP Protein _ 91360ES

YeasenSKU: 91360ES50
Size: 50 μg
Harga:
$265.00

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Keterangan

Basic Fibroblast Growth Factor (bFGF) is a member of the Fibroblast Growth Factor (FGF) family and serves as a growth factor and signaling protein encoded by the FGF2 gene. FGF family members exhibit broad mitogenic and cell survival activities, participating in diverse biological processes including embryonic development, cell growth, morphogenesis, tissue repair, as well as tumor growth and invasion. In normal tissues, bFGF is present in the basement membrane and the sub-endothelial extracellular matrix. bFGF stimulates and regulates the proliferation and differentiation of various mesoderm- and neuroectoderm-derived cells, such as vascular endothelial cells, epithelial cells, myoblasts, osteoblasts, and glial cells, playing a vital role in embryonic development and tissue healing. Furthermore, bFGF (FGF-2) is an essential component in all embryonic and induced pluripotent stem cell culture media for maintaining pluripotency.

Components

Components No.

Name

91360ES10

91360ES50

91360ES80

91360

Recombinant Human FGF basic/FGF2/bFGF GMP Protein

10 μg

50 μg

1 mg

Specifications

Synonyms

bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2

Source

Human bFGF Protein is expressed from E.coli without tag.

Sequence

Ala 144 - Ser 288.

Molecular Weight

Approximately 16.29 kDa.

Accession

P09038

Biological Activity

Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

＜0.01 EU/μg protein as determined by LAL method.

Sterility

Negative.

Mycoplasma

Negative.

Vitro Virus

Negative.

Host Cell Protein

Less than 0.5 ng/μg when tested by ELISA.

Host Cell DNA

Less than 20 ng/mg when tested by qPCR.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.  

Physical Appearance

Lyophilized Powder

Storage

This product should be stored at -25~-15℃ for 3 years. After reconstitution, store at -85 to -65°C. Valid for 3 months.

Reconstitution Instructions

It is recommended to reconstitute Human bFGF in sterile distilled water to a concentration of 0.1–1.0 mg/mL. Avoid repeated freeze-thaw cycles whenever possible.

Notes

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves，for your safety.

Figure

SDS-PAGE 

Figure 1. Human FGF2/bFGF GMP Protein on SDS-PAGE under reduced condition. The purity is greater than 95%

Figure 1. Human FGF2/bFGF GMP Protein on SDS-PAGE under reduced condition. The purity is greater than 95%

Cell Based Assay

Figure 2. Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).

Figure 2. Human bFGF GMP Protein stimulates proliferation of murine balb/c 3T3 cells. The specific activity of Human bFGF GMP Protein is >8.0 x 10^5 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712).

Documents:

Safety Data Sheet

91360_MSDS_HB260716

Manuals

91360_Manual_HB20260716

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