Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES
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Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES

Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES

YeasenSKU: 20543ES10
Size: 10 mg
Harga:
$90.00

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Keterangan

Protein A is a cell wall component produced by several strains of Staphylococcus aureus, consisting of a polypeptide chain and containing almost no carbohydrates. This product is produced in Escherichia coli and functions similarly to natural protein A. It consists of five IgG binding domains E-D-A-B-C arranged in series, with strong specific affinity for the Fc fragment of IgG. Protein A, as an affinity ligand, is coupled to the matrix and can specifically bind to antibody molecules in the sample, allowing other impurities to flow through and exhibiting high selectivity. Commonly used for purification of monoclonal antibodies or polyclonal antibodies. Protein A binds to human, mouse, and rat IgG subclasses (e.g. human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c), as 
well as total IgG from rabbits, guinea pigs, pigs, dogs, and cats.

Components

Components No.

Name

20543ES10

20543ES60

20543

RNase A  (10 mg/mL)

10 mg

100  mg

Product Properties

Synonyms

Recombinant protein A alkali-tolerant

Source

E.coli

 

Molecular Weight

Approximately 26.7 kDa, a single non-glycosylated polypeptide chain containing 237amino acids

 

 

 

Amino Acid Sequence

AQGTVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKLND

AQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKLN  DAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKL  NDAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKK LNDAQAPKC

Concentration for  1  unity  OD at 280 nm

 

4.4 mg/mL

Physical Appearance

> 97 % by SDS-PAGE and HPLC analyses.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Endotoxin Level

<0.1 EU /1 μg of the protein by the LAL method.

 

Formulation

A 0.2 µm filtered solution in 20 mM PB,400 mM NaCl, pH7.4 or lyophilized from a 0.2 µm filtered solution in 20 mM PB,400 mM NaCl, pH7.4

Reconstitution

Dissolve in distilled water or saline.

Storage

This product should be stored at -25~ -15℃, valid for 12 months. Avoid repeated freeze-thaw cycles.

Notes

1. Please operate with lab coats and disposable gloves ，for your safety.

2. This product is for research use only.

Appendix

Table 1. Summary of Binding Abilities of Proteins A, G, and L to Ig from Different Species

Table 1. Summary of&nbsp;Binding Abilities of&nbsp;Proteins&nbsp;A,&nbsp;G,&nbsp;and&nbsp;L&nbsp;to&nbsp;Ig&nbsp;from&nbsp;Different&nbsp;Species

Documents:

Safety Data Sheet

20543-MSDS-HB250701

Manuals

20543-Manual-Ver.EN20250701

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