Keterangan
The HieffTM Gel Extraction Kit adopts HieffTM DNA Column G1 and efficient sol system, which is suitable for recovering high-quality DNA fragments with the length of 100 bp-40 kb from TAE or TBE agarose gel. In the recovery process, toxic organic substances such as phenol and chloroform do not need to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The operation is simple, the sol time is short, the volume of added sol solution is small, and the whole purification process can be completed in 20 minutes. The HieffTM DNA Column G1 in the kit can selectively adsorb up to 8 μg of DNA fragments, and does not adsorb enzymes, mineral oil and other impurities. The obtained DNA has high purity and can be directly used for subsequent experiments such as enzyme digestion, ligation, transformation, sequencing and library construction etc.
Features
- Wide Application Range: Efficiently recovers DNA fragments from 50 bp to 15 kb.
- High Recovery Efficiency: Binds up to 8 μg of DNA per column.
- Fast & Efficient Workflow: Purify DNA in just 10 minutes with up to 85% recovery.
Components
|
Components No. |
Name |
19101ES08 |
19101ES50 |
19101ES70 |
|
5 T |
50 T |
200 T |
||
|
19101-A |
HieffTM DNA Column G1 |
5 |
50 |
200 |
|
19101-B |
2 mL Collection Tube G1 |
5 |
50 |
200 |
|
19101-C |
AC Buffer G1 |
0.5 mL |
5 mL |
20 mL |
|
19101-D |
BD Buffer G1 |
2 mL |
20 mL |
80 mL |
|
19101-E |
Wash Buffer* |
1.3 mL |
13 mL |
50 mL |
|
19101-F |
Elution Buffer |
1 mL |
10 mL |
20 mL |
Shipping and Storage
This product should be stored at room temperature away from light, valid for 2 years.
Figures
Superior DNA Recovery Efficiency
Figure 1. Agarose Gel Electrophoresis of Recovered DNA Fragments (0.5 kb & 1 kb)
DNA fragments were recovered and analyzed via gel electrophoresis. Yeasen 19101 demonstrated greater band intensity and higher recovery efficiency compared to competitor T.
FAQ
Q: How long will the operation take?
A: Simple operation: The purification process can be completed in just 20 minutes.
Q: What is the recovery rate of the glue recycling kit?
A: The recovery rate is over 60%, and it can reach up to 90%.
Q: Can the rubber waste be directly recycled into the PCR solution?
A: Yes. The solution should still contain the sol solution, but instead of being placed in a 50-60℃ water bath， the other steps remain the same. The ratio is 50 uL of PCR stock solution for the system, and add 200 uL of sol solution. For smaller fragments, add isopropanol according to the instructions. However， it is more recommended to use a dedicated PCR product purification kit (item number：19106ES).
Q: Can the nucleic acids in the polyacrylamide gel be recovered?
A: No, for the PAGE gel, it is recommended to use a dedicated kit. However, Yeasen currently does not have such products.
Q: Why is the solution purple after adding the solubilizing solution BD? Is this normal?
A: If the solution appears orange or purple, it is recommended to add 10 μL of 3M sodium acetate (with a pH value of 5.2) solution for mixing. The color of the mixture then turns yellow.
Subsequently, further experiments were conducted, and the yellow color indicated the optimal pH value for DNA binding.
Q: The recovery rate is low. Do you have any suggestions?
A: 1) During electrophoresis, use fresh electrophoresis solution. Using old solution will cause an increase in pH value, reducing the adsorption force between DNA and the membrane.
2) When cutting the gel, while ensuring the integrity of the target fragment, try to minimize the volume of the gel and reduce the exposure time under the ultraviolet light.
3) Observe the color of the mixture after adding the sol gel solution. If the color of the solution is orange or purple, add 10 μL of 3M sodium acetate (pH 5.2) solution and mix. Continue with the subsequent experiments once the color of the mixture turns yellow.
4) The sol must be completely homogeneous. You can appropriately extend the time of the water bath and the number of upside-down operations.
5) Before adding the elution solution, you can appropriately extend the drying time at room temperature.
6) Preheat the elution solution at 70℃, which can increase the recovery rate. When adding the elution solution, make sure to pour it into the center of the membrane and prolong the incubation time at room temperature.
7) For the recovery of small fragments, more sol solution can be added. After the sol is dissolved, 0.3 times the volume of isopropanol can be added， which can significantly increase the recovery rate. Additionally， using elution solution preheated at 70℃ can also enhance the recovery yield.
Documents:
Safety Data Sheet
Manuals
19101_Manual_Ver.EN20250825.pdf
Citations & References:
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