Keterangan
The Hieff™ Pro Soil/Stool DNA Kit is designed to extract high-purity DNA from 250 to 500 mg of soil samples. Utilizing superparamagnetic bead purification technology, this method eliminates the need for toxic phenol-chloroform extraction, offering a simple and convenient workflow. The extracted DNA is ready for direct use in downstream applications such as PCR, qPCR, microarray analysis, and Sanger or Next-Generation Sequencing (NGS). When paired with automated magnetic bead extraction instruments, the kit enables high-throughput nucleic acid extraction.
Features
Broad Applicability: Compatible with 250–500 mg soil and fecal samples.
Deep Impurity Removal: Effectively eliminates complex inhibitors from challenging soil matrices.
High-Purity Nucleic Acids: Yields DNA ready for direct use in PCR, qPCR, and NGS/Third-Generation Sequencing.
High-Throughput Compatibility: Supports automated instruments for rapid, batch processing.
Components
|
Category |
Components No. |
Name |
18527ES48 (48 T) |
|
Part Ⅰ |
18527-A |
Proteinase K |
1.1 mL/tube × 1 |
|
Part II |
18527-B |
Magnetic Bead Suspension |
1.1 mL/tube × 1 |
|
18527-C |
Lysis Buffer |
30 mL/bottle × 1 |
|
|
18527-D |
Grinding Tube |
8 tubes/bag × 1 bag |
|
|
18527-E |
Lysis Enhancer SE |
3 mL/bottle × 1 |
|
|
18527-F |
Precipitation Solution |
7 mL/bottle × 1 |
|
|
18527-G |
Lysis Buffer LB |
30 mL/bottle × 1 |
|
|
18527-H |
Deproteinization Solution PL* |
40 mL/bottle × 1 |
|
|
18527-I |
Wash Buffer AW* |
40 mL/bottle × 1 |
|
|
18527-J |
Wash Buffer BW* |
40 mL/bottle × 1 |
|
|
18527-K |
Elution Buffer DE |
6 mL/bottle × 1 |
[Note]: Add 6.3 mL isopropanol into the bottle of 18527-H Deproteinization Solution PL.
Add 12 mL isopropanol into the bottle of 18527-I Wash Buffer AW.
Add 34 mL absolute ethanol into the bottle of 18527-J Wash Buffer BW.
Storage
Store Part I at 2–8°C, valid for 18 months.
Store Part II at room temperature, valid for 18 months.
Documents:
Safety Data Sheet
Manuals
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Pertanyaan
FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
Aplikasi tertentu mungkin memerlukan hak kekayaan intelektual pihak ketiga tambahan.