Keterangan
The RT-LAMP Fluorescent Assay Kit (Bst Plus) is a kit capable of isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve highly efficient, rapid, and highly specific detection reactions, and supports room-temperature reaction system preparation.
Features
- Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
|
Components No. |
Name |
16729ES60 |
16729ES80 |
|
16729-A |
4×LAMP Buffer |
600 µL |
6 mL |
|
16729-B |
Bst Plus DNA Polymerase |
100 µL |
1 mL |
|
16729-C |
RT Enzyme mix |
50 µL |
500 µL |
|
16729-D |
25×Fluorescent Dye |
100 µL |
1 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Application
Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow.
2. Broad Compatibility: DNA Pathogen Detection&RNA Pathogen Detection
Figure 2. Broad Pathogen Detection Compatibility.
(A) DNA pathogen detection: Reliable amplification observed across multiple DNA virus targets.
(B) RNA pathogen detection: Effective detection of RNA viruses with high sensitivity and specificity.
3. High Specificity
Figure 3. High Specificity of RT-LAMP Assay (Cat#16729) Compared to Supplier A*
Specificity was evaluated using both DNA and RNA templates, including positive targets and non-target (negative) controls. The RT-LAMP assay (Cat#16729) showed no false positives and clear separation between positive and negative samples, outperforming Supplier A* by avoiding non-specific amplification.
4. Strong stability
Figure 4. Stability Evaluation of the RT-LAMP System.
(A) Freeze-thaw stability: Performance remains consistent after 20 freeze-thaw cycles.
(B) Thermal accelerated stability: Maintains amplification efficiency after 7 days of storage at 37°C.
Documents:
Safety Data Sheet
Manuals
16729_Manual_Ver.EN20250708.pdf
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FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
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