Keterangan
This product is a type II restriction endonuclease, a recombinant protein encoded by the BspQI gene from Bacillus sphaericus expressed in E. coli. It recognizes the sequence 5'-GCTCTTCN1/N4-3' and is mainly used for digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends, obtaining specific sticky ends.
Applications
Digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends.
Digesting DNA to obtain specific sticky ends.
Specifications
|
Recognition site |
5'-GCTCTTC(N)↓-3' 3'-CGAGAAG(N)4↑-5' |
|
Concentration |
10 U/µL |
|
Definition of Unit |
The amount of enzyme required to digest 1 µg of λDNA in a 50 µL reaction volume at 50°C within 1 hour is defined as one unit (U) of activity. |
|
Reaction conditions |
React at 50℃ for 60 min |
Components
|
Name |
15202ES76 |
15202ES86 |
15202ES92 |
|
BspQI (10 U/μL) |
50 μL |
250 μL |
1 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 1 year.
Documents:
Safety Data Sheet
Manuals
Pembayaran & Keamanan
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Pertanyaan
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FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
Aplikasi tertentu mungkin memerlukan hak kekayaan intelektual pihak ketiga tambahan.