Keterangan
This kit integrates a visible-light LAMP indicator into the formulation. The reaction buffer contains dATP, dCTP, dGTP, and dUTP—together with additional components essential for Bst II DNA Polymerase amplification—as well as a pH-sensitive indicator. The kit also provides glycerol-free Bst II DNA Polymerase, UDGase, and reverse transcriptase (RT enzyme), and is supplied with a lyophilization protectant, enabling direct preparation of lyophilized reaction systems on a standard freeze-dryer. The reagent delivers high amplification efficiency and sensitivity, with amplification results readable by the unaided eye: positive reactions turn orange-yellow while negative reactions remain magenta, providing strong visual contrast.
Product Information
|
Product Name |
Cat. No. |
Size |
|
RT-LAMP pH Sensitive Dye Chromogenic Version Freeze-Dryable Reagent |
13920ES60 |
100 T |
|
13920ES80 |
1,000 T |
|
|
13920ES92 |
10,000 T |
Product Components
|
Component No. |
Component Name |
13920ES60 (100 T) |
13920ES80 (1,000 T) |
13920ES92 (10,000 T) |
|
13920-A |
2.5× pH Sensitive Reaction Buffer |
1 mL |
10 mL |
100 mL |
|
13920-B |
Glycerol-free RT Enzyme Mix |
50 μL |
500 μL |
5 mL |
|
13920-C |
Glycerol-free Bst Enzyme |
30 μL |
300 μL |
3 mL |
|
13920-D |
Lyophilization Protectant |
500 μL |
5 mL |
50 mL |
Applications
Features
• One-step isothermal RNA amplification: visual colorimetric readout eliminates the need for electrophoresis or fluorescence instrumentation.
• Rapid turnaround: pathogen status can be determined in as little as 30 minutes after sample loading.
• Flexible workflow: a constant-temperature water bath or standard PCR thermal cycler (60–65°C) is sufficient—no specialized LAMP instrument required.
• Lyophilization-ready: glycerol-free enzymes and a dedicated lyophilization protectant enable direct preparation of room-temperature-stable lyophilized kits.
• High contrast endpoint: orange-yellow (positive) versus magenta (negative) for unambiguous visual interpretation, suitable for high-throughput population screening.
Storage
Part I — Components 13920-A and 13920-D [2.5× pH-Sensitive Reaction Buffer / Lyophilization Protectant] are shipped on dry ice and should be stored at -25 to -15°C with a shelf life of 6 months.
Part II — Components 13920-B and 13920-C [Glycerol-free RT Enzyme Mix / Bst Enzyme] are shipped with ice packs and should be stored at 2 to 8°C with a shelf life of 3 months.
FAQ
Q: Can 13920ES be used directly without lyophilization?
Yes. Lyophilization is optional; the kit may be used directly as a liquid formulation without any loss of performance.
Q: The 13920ES protocol includes an 85°C inactivation step for 5 minutes. Should the tube be removed after this inactivation step, or after the 30-minute reaction at 60–65°C, for color observation?
A: The tube should be removed for color observation only after the 85°C inactivation step (5 minutes) has been completed.
Documents:
Safety Data Sheet
Manual
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Pertanyaan
FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
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