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Hieff™ LongSeq DNA Endprep&dA-Tail Module is a DNA end-repair and A-tailing module developed for the third-generation Nanopore sequencing platform. This kit features directionally engineered enzymes and optimized buffers, enabling efficient repair of various samples such as 100 ng-1.5 µg gDNA and amplicons. Moreover, purification is not required after end-repair and A-tailing; the product can be directly used for Barcode ligation, making the experimental workflow more convenient.
Features
Optimized for LongSeq Platforms: Specifically designed for end-repair and dA-tailing in Nanopore sequencing workflows.
High Efficiency: Superior enzymatic performance.
Wide Input Range: 100 ng – 1.5 μg (gDNA, amplicons, etc.).
Components
|
Components No. |
Name |
13302ES08 |
13302ES24 |
13302ES96 |
|
|
8 T |
24 T |
96 T |
|||
|
13302-A |
|
Endprep Buffer |
56 μL |
168 μL |
672 μL |
|
13302-B |
|
Endprep Enzyme |
24 μL |
72 μL |
288 μL |
Figure
1. Human Genome Sequencing Case
2. Microbial genome Sequencing Case
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