The CpG cytosines on the sequence of Hieff™ Methylated PUC19 DNA are fully methylated, which can be used as a known "positive" standard to evaluate the efficiency and accuracy of the experimental workflow. After sequencing, theoretically the C on CpG will not be converted. By calculating how many C have been converted to T, the over-conversion efficiency of bisulfite in this experiment can be evaluated, ensuring the reliability of subsequent analysis. The full length of the PUC19 DNA sequence is 2686 bp.
Specifications
|
Product Name |
Catalog No. |
Size |
|
Hieff™ Methylated PUC19 DNA |
12233ES50 |
50 ng |
Storage
The product should be stored at -25°C to -15°C for 2 years.
Notes
1. As a "spike-in" (positive control), it is recommended to spike into real samples at a ratio of 0.1%–1% and proceed with the entire experimental workflow together.
2. For your safety and health, please operate with lab coats and disposable gloves.
3. This product is for research use only.
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FAQ
Produk ini hanya untuk keperluan penelitian dan tidak ditujukan untuk penggunaan terapeutik atau diagnostik pada manusia atau hewan. Produk dan konten dilindungi oleh paten, merek dagang, dan hak cipta milik
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