Product Alias

MHC, HLA-A, Peptide Ready

Species

Human

Expression sequence

Gly25-Thr305(HLA-A*24:02) and Ile21-Met119(B2M)

Accession

AAA59600.1(HLA-A*24:02)&P61769(B2M)

Label

C-His-Avi

Expression system

HEK293

Molecular weight

The protein has a predicted MW of 48.30 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Preparation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized Biotinylated Human Peptide Ready HLA-A*24:02&B2M Monomer, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.