विवरण
The Hieff™ Bacterial DNA Kit is designed for the extraction of genomic DNA from Gram-positive and Gram-negative bacteria. The silica-based matrix used in the spin column is a proprietary novel material. Combined with our unique buffer formulation, it maximizes the removal of impurities such as cellular metabolites and proteins. The extracted DNA is of high purity and stable quality, making it suitable for various downstream applications, including restriction enzyme digestion, PCR, and library construction.
Application
Broad Applicability: Suitable for both Gram-positive and Gram-negative bacteria.
High DNA Purity: Free from protein and RNA contamination, ready for direct use in downstream applications such as PCR, restriction enzyme digestion, and qPCR.
Safe and Eco-friendly: Eliminates the need for toxic organic solvents like phenol and chloroform during extraction.
Components
|
Category |
Components NO. |
Name |
18806ES08 |
18806ES50 |
18806ES70 |
|
Part I |
18806-A |
Proteinase K (20 mg/mL) |
100 μL |
1 mL |
1 mL×4 |
|
Part II |
18806-B |
DNA Binding Column (Hieff™ DNA Column B1) |
5 |
50 |
200 |
|
18806-C |
2 mL Collection Tube B1 |
5 |
50 |
200 |
|
|
18806-D |
Buffer AC (AC Buffer B1 |
500 μL |
5 mL |
20 mL |
|
|
18806-E |
Lysis Buffer LB(LB Buffer B1) |
3 mL |
30 mL |
120 mL |
|
|
18806-F |
Binding Buffer BD (BD Buffer B1) |
1 mL |
10 mL |
40 mL |
|
|
18806-G |
Protein Removal Buffer PL* (PL Buffer B1*) |
1.6 mL |
16 mL |
64 mL |
|
|
18806-H |
Wash Buffer W* (Wash Buffer*) |
1.3 mL |
13 mL |
50 mL |
|
|
18806-I |
Elution Buffer |
1 mL |
10 mL |
20 mL |
Storage
This product should be stored at -85~-65℃ for 2 years.
Figure
1. Higher Yield
Figure 1. Comparison of gDNA extraction performance between Kit 18806.
Four aliquots of 2 mL E. coli suspension were processed using Kit 18806(Y1/Y2) and a comparable product from Brand T(T1/T2), with gDNA eluted in 50 μL ddH₂O. Subsequently, 5 μL of each eluate was analyzed by gel electrophoresis. The results demonstrate that Kit 18806 yields a higher amount of gDNA with superior integrity compared to Brand T.
FAQ
Q1: No PCR amplification bands were observed using the extracted DNA. What could be the cause?
A1: This issue may be caused by low DNA yield, DNA degradation, or low DNA purity. DNA extraction involves two main stages: sample preparation and DNA extraction. Key factors include the freshness of the sample, the method of cell lysis and nucleic acid release, and the binding capacity of the column.
Q2: Why is the DNA yield low?
A2:
(1) Poor quality of the starting material or insufficient sample amount.
(2) Incomplete cell wall disruption or lysis. For plant samples, ensure thorough homogenization and grinding. Avoid improper storage of the lytic enzymes provided in the kit. Pre-warming the elution buffer or performing multiple passes through the column can also help.
Q3: Why is the DNA degraded?
A3:
(1) The starting material is not fresh, or it has undergone repeated freeze-thaw cycles.
(2) Harsh handling during the extraction process may cause mechanical shearing of the DNA.
Solutions: Use fresh starting materials whenever possible and store samples at low temperatures to avoid repeated freeze-thaw cycles. After grinding in liquid nitrogen or tissue homogenization, handle the samples gently during subsequent steps. Avoid repeated freeze-thaw cycles of the DNA elution buffer.
Q4: Why is the DNA purity low?
A4:
(1) Contamination by residual proteins or RNA.
(2) Residual ethanol. Ensure that absolute ethanol is added to the Wash Buffer as instructed, and prevent the ethanol from evaporating prematurely. Perform an additional dry spin (centrifugation of the empty column) to ensure that all residual ethanol is completely removed.
Documents:
Safety Data Sheet
Manuals
Publications
[1] Immune checkpoint therapy-elicited sialylation of IgG antibodies impairs antitumorigenic type I interferon responses in hepatocellular carcinoma.
Journal: IMMUNITY | DOI: 10.1016/j.immuni.2022.11.014 | IF: 43.47
[2] Enzymatic bionanocatalysts for combating peri-implant biofilm infections by specific heat-amplified chemodynamic therapy and innate immunomodulation.
Journal: DRUG RESISTANCE UPDATES | DOI: 10.1016/j.drup.2022.100917 | IF: 24.3
[3] Spoilage potential of Bacillus isolates from preserved furu rou (sufu-braised streaky pork) prepared dish and their impact on volatile organic compounds during storage.
Journal: LWT-FOOD SCIENCE AND TECHNOLOGY | DOI: 10.1016/j.lwt.2026.119373 | IF: 7.1
[4] Hijacking competitor-derived signals: RcsB/C drives Lysobacter enzymogenes to exploit farnesol for enhanced antifungal capacity.
Journal: APPLIED AND ENVIRONMENTAL MICROBIOLOGY | DOI: 10.1128/aem.00304-26 | IF: 4.2
[5] Nanocoordinated Zn-Baicalein: Merging triple mechanisms with photothermal action for enhanced bacterial eradication.
Journal: DYES AND PIGMENTS | DOI: 10.1016/j.dyepig.2026.113641 | IF: 3.9
[6] Dual-Enzyme Cofactor Recycling Drives Efficient 9α-OH-AD Biosynthesis.
Journal: KOREAN JOURNAL OF CHEMICAL ENGINEERING | DOI: 10.1007/s11814-026-00734-y | IF: 3.4
[7] Detection of Oral Pathogens and Potential Virulent of Dominant Klebsiella pneumoniae.
Journal: ORAL DISEASES | DOI: 10.1111/odi.70275 | IF: 3.1
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उपवास
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