When recombinant protein expression becomes a systems problem, a matched kit beats a best-in-class reagent—every time.
In antibody discovery, antigen production, vaccine R&D, and structural biology, transient transfection of HEK293 cells has become the go-to route to recombinant protein. Compared with the 3–6 months required to build a stable cell line, transient expression delivers "plasmid → transfection → protein harvest" in a matter of days—an unbeatable fit for the speed and flexibility early-stage research demands.
But speed has a trade-off: low yield. The ceiling of transient expression is rarely set by any single "best" reagent. It's set by how well the entire transfection–culture–feed workflow works together as a system. That's the "last mile" the industry has struggled to solve—until now.
One Kit, One System: No Optimization Required
Built on Yeasen's proprietary transfection reagent and media R&D platform, the Hieff Trans™ UltraProFect-HEK293 Transfection Reagent Kit (Cat. #40829ES) is purpose-designed for high-titer transient protein expression in suspension HEK293 cells—covering secreted proteins, membrane proteins, cytokines, and antibodies alike.
Instead of mixing reagents from multiple vendors and hoping they're compatible, you get a fully integrated system: transfection reagent, dilution buffer, serum-free medium, and feeds—co-developed and co-validated on a single platform. Open the box, transfect, and harvest. No cross-vendor optimization, no batch-to-batch compatibility surprises.
Kit contents (Cat. #40829ES80, 1000 mL format):
|
Component No. |
Component |
Size |
Function |
|
40829-A |
UltraProFect Transfection Reagent |
3 × 1 mL |
High-efficiency, low-toxicity transfection reagent designed for high-density 293 suspension systems |
|
40829-B |
TransBuffer Dilution Buffer |
100 mL |
Optimizes complex formation conditions for stable, reproducible transfection efficiency |
|
40829-C |
HEK293-PRO Serum-Free Medium |
1000 mL |
Chemically defined medium supporting high-density suspension growth |
|
40829-D |
Feed YS11 |
300 mL |
Extends the expression window and continuously supports high output |
|
40829-E |
Feed YS12 |
100 mL |
Synergistically optimizes protein expression in the late stage |
Five components, one complete workflow spanning cell culture → plasmid transfection → feed-driven enhancement—eliminating the hassle of multi-supplier sourcing and the uncertainty of cross-system incompatibility.
Core Advantages: 2× Higher Titers
In side-by-side benchmarking against leading brand, the UltraProFect-HEK293 system delivered 2–3× higher protein expression across multiple targets:
Case Study 1 — Antigen protein: Day 7 titer of 452 mg/L vs. 148 mg/L for a comparable product — a 3.05× improvement.
Case Study 2 — Antibody protein: Day 7 titer of 662 mg/L vs. 303 mg/L for a comparable product — a 2.18× improvement.
Engineered for Suspension Culture, Ready to Scale
The kit is deeply optimized for suspension systems such as Expi293 and 293F, scaling smoothly from shake flasks to bioreactors. A process validated at bench scale behaves predictably at scale-up—fewer surprises, no "it worked in the flask" moments.
Transient expression is no longer a single-reagent race—it's a whole-system race. By integrating transfection, culture, and feeding into one validated, ready-to-use workflow, the Hieff Trans® UltraProFect-HEK293 Kit gives you 2× the titer with zero optimization overhead—so you spend your time on the science, not the process.
Get to protein faster. Get to answers sooner. Start with one box.
Product Information
|
Cat. No. |
Product name |
Size |
|
40829ES |
1000 mL |
|
|
40830ES |
1 mL 10 mL 100 mL |