Principles and Troubleshooting of Plasmid Extraction

Principles and Troubleshooting of Plasmid Extraction

Plasmid extraction is a fundamental molecular biology technique used to isolate plasmid DNA from bacterial cells. Depending on the required yield a...
Principles and Troubleshooting of Plasmid Extraction

Principles and Troubleshooting of Plasmid Extraction

Plasmid extraction is a fundamental molecular biology technique used to isolate plasmid DNA from bacterial cells. Depending on the required yield a...
Don’t Know How Much cDNA to Use? Still Measuring cDNA Concentration?

Don’t Know How Much cDNA to Use? Still Measuring cDNA Concentration?

Quantitative PCR (qPCR) is a widely used technique in molecular biology, often perceived as straightforward. However, successful qPCR experiments h...
Are You Choosing the Right Method for Nucleic Acid Quantification?

Are You Choosing the Right Method for Nucleic Acid Quantification?

Nucleic acid extraction is a routine procedure in most molecular biology labs. The extracted DNA or RNA is often used in downstream applications su...
Troubleshooting Nucleic Acid Gel Electrophoresis—Solved in One Go!

Troubleshooting Nucleic Acid Gel Electrophoresis—Solved in One Go!

Introduction Agarose gel electrophoresis is likely one of the first experiments you performed in the lab. Today, let's take a deep dive into this...
GMP-Grade Recombinant Human bFGF/FGF2 — A Key Factor in Stem Cell Culture

GMP-Grade Recombinant Human bFGF/FGF2 — A Key Factor in Stem Cell Culture

01 Overview Basic Fibroblast Growth Factor (bFGF), also known as Fibroblast Growth Factor-2 (FGF-2), is a member of the FGF family, which include...
Why Does Your Melt Curve Look So Strange?

Why Does Your Melt Curve Look So Strange?

Troubleshooting Abnormal Melt Curves in qPCR In qPCR experiments, melt curves are essential for ensuring product specificity and data accuracy. Abn...
Why Do Amplification Curves Show Spikes?

Why Do Amplification Curves Show Spikes?

Perfect amplification curves follow a predictable pattern, but anomalies can appear in all sorts of strange shapes. One common issue, especially wi...
Free Guide: Top 5 PCR Optimization Tips

Guide gratuit: les 5 meilleurs conseils d'optimisation de la PCR

La PCR (Polymerase Chain Reaction) est une technique fondamentale de la biologie moléculaire, largement utilisée pour le clonage de gènes, les test...
PCR, Nucleic Acid Electrophoresis, Laboratory  Techniques  Handbook

PCR, électrophorèse en acide nucléique, manuel des techniques de laboratoire

Chapitre 1 : La technologie PCR 1.1 Principes de la PCR La PCR (Polymerase Chain Reaction), ou réaction en chaîne par polymérase, fait ré...
Hieff Clone Universal II One Step Cloning Kit ——1–7 fragment one-step cloning

Hieff Clone Universal II Kit de clonage d'un pas —— 1–7 Fragment Clonage en une étape

Êtes-vous toujours gêné par la faible efficacité de la recombinaison homologue de fragments multiples ou super longs et par le petit nombre de colo...
YEASEN Hieff UNICON™ Series Dye-based Fluorescent Quantitative Reagents Aid in Beautiful Curves

Les réactifs quantitatifs fluorescents à base de colorants à base de colorants Hieff Unicon ™ aident à de belles courbes

La PCR quantitative fluorescente est une expérience extrêmement sensible qui nécessite un haut degré de précision dans la mise en œuvre par le pers...
Hieff Canace™ Series High-fidelity - Rapid High-fidelity, 5 seconds/kb, ensures fidelity and speed!

Série Hieff Canace ™ Haute fidélité - Haute fidélité rapide, 5 secondes / kb, assure la fidélité et la vitesse!

Lors de l'utilisation d'enzymes traditionnelles de haute fidélité pour la PCR, il est inévitable de rencontrer des problèmes épineux. Par e...
From RNA to cDNA, YEASEN Hifair™ Reverse Transcription 5-Minute Series Products Make a Quick Breakthrough!

De l'ARN à l'ADNc, la transcription inverse de la série de 5 minutes Yeasen Hifair ™ fait une percée rapide!

La transcription inverse est le processus de conversion de l'ARN en ADN complémentaire (ADNc), qui est médié par l'enzyme transcriptase inv...

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