Cellule
Protocol for Transfection of Primary Bone Marrow-Derived Macrophages (BMDMs)
1. Experimental Materials
BMDM Cells
Target plasmid DNA (Concentration ≥ 1000 ng/μL)
Hieff TransTM Booster DNA&RNA Transfection Reagent...
Beyond Liposomes: The Next Generation Booster DNA/RNA Transfection Reagent (Get Free Trial at the Bottom of the Page)
High toxicity, low efficiency, and poor compatibility have long been the three major challenges in cell transfection.Traditional transfey. For prim...
Translation: Understanding PEI Transfection Reagents
Background of PEI Transfection Reagents
About 50 years ago, Theodore Friedmann and Richard Roblin proposed using “good” exogenous DNA to replace “d...
A Comprehensive Guide to Phalloidin Staining for Cytoskeleton
Abstract:
The cytoskeleton is mainly composed of microfilaments, microtubules, and intermediate filaments. Microfilaments are primarily made of act...
Common Questions in Organoid Culture
Organoid culture has become increasingly popular in recent years. Organoids are derived from adult or pluripotent stem cells differentiated in vitr...
Bioluminescence Imaging: A Powerful Tool for Real-Time, In Vivo Research
1. Introduction to the Technical Principle
1) Bioluminescence imaging (BLI) in live animals involves the expression of a reporter gene (such as l...
Common Problems and Solutions in Annexin V-Based Flow Cytometry Apoptosis Assays
Zhang A, Pan S, Zhang Y, et al. Carbon-gold hybrid nanoprobes for real-time imaging, photothermal/photodynamic and nanozyme oxidative therapy. The...
What to Do When Mitochondrial Labeling Goes Wrong? Common Abnormalities and Solutions
Mitochondria are involved in various cellular processes, including autophagy, calcium homeostasis, immune response, signal transduction, and apopto...
How to Label Exosomes? High-Efficiency Exosome Fluorescent Labeling FAQ
Exosomes are membrane-bound vesicles actively secreted by cells, typically 30–150 nm in diameter. They contain various biomolecules derived from ho...
Cell Viability and Proliferation: Unlocking the Code of Cellular "Vitality"
As the basic unit of life, the vitality of cells directly reflects an organism’s health status and disease progression. Assessing cell viability is...
Revisiting Technical Challenges in Tumor Cell Invasion and Migration Assays: Causes and Optimization Strategies
Inhibited Migration to the Lower Chamber
This issue may result from an insufficient chemotactic gradient or the absence of effective chemoattracta...
Common Issues in Liposome Transfection
Transfection is one of the most widely used techniques in cell-based experiments. It involves introducing exogenous nucleic acids into cells using ...