Validated, high-efficiency transfection protocols for a wide range of tumor cell lines—helping you achieve optimal results with minimal optimization, right from the first attempt. We provide dedicated experimental protocols for commonly used tumor cells, covering key aspects including cell growth, culture conditions, passaging procedures, and critical steps for highly efficient transfection.

These optimized protocols offer detailed, step-by-step guidance to achieve high transfection efficiency under low cytotoxicity conditions. Validated by numerous academic institutions and research centers worldwide, our solutions are designed to meet real-world experimental needs and enable faster, more reliable results.

Optimized Transfection Protocols for Tumor Cells

Cell Name

Cell name abbreviation

Nucleic Acid Type

Human T-lymphoblastic leukemia cells

Jurkat

DNA

Human cervical cancer cells

Hela

DNA

Mouse colon cancer cells

MC38

DNA

Human gastric adenocarcinoma cells

AGS

DNA

Human hepatocellular carcinoma cells

HepG2

DNA

Human colon cancer cells

HCT-116

siRNA

Human gastric cancer cells

HGC-27

siRNA

Human bladder transitional cell carcinoma cells

T24

DNA

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