Descripción
Hieff NGSTM MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.
Features
- Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.
- Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).
- High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.
- Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.
Components
|
Components No. |
Name |
12257ES12 |
12257ES24 |
12257ES96 |
|
12257-A |
Hybridization Buffer |
36 μL |
72 μL |
288 μL |
|
12257-B |
Human Probe Mix (rRNA & ITS/ETS) |
24 μL |
48 μL |
192 μL |
|
12257-C |
RNase H Buffer |
36 μL |
72 μL |
288 μL |
|
12257-D |
RNase H |
4 μL |
48 μL |
192 μL |
|
12257-E |
DNase I Buffer |
330 μL |
660 μL |
2x1320 μL |
|
12257-F |
DNase l |
30 μL |
60 μL |
240 μL |
Applications
- Gene Expression Research
- Alternative splicing analysis
- Detection and discovery of LncRNA(Non coding RNA)
- dentification of selective polyadenylation sites
- Fusion gene detection
Storage
This product should be stored at -25~-15℃ for 12 months.
Pago y seguridad
Su información de pago se procesa de forma segura. No almacenamos detalles de la tarjeta de crédito ni tenemos acceso a la información de su tarjeta de crédito.
Consulta
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
Yeasen se dedica a la ciencia ética y cree que nuestra investigación debe abordar cuestiones críticas al tiempo que garantiza la seguridad y los estándares éticos.