The Lentivirus Physical Titer qPCR Detection Kit is a kit designed to quantitatively detect the physical titer of lentivirus (total number of viral particles) using the conserved region (LTR) in the genome of HIV-1-derived lentiviral vectors as a target.

This kit adopts the principle of fluorescence quantitative PCR by probe method to rapidly and specifically detect lentivirus RNA. The widest linear range can reach 5 copies/μL~5×108 copies/μL, with a detection limit as low as 0.5 copies/μL. And it contains the Lentivirus RNA Control (RNA quantitative reference product). The kit needs to be used together with the the Residual DNA Sample Preparation Kit (Cat# 18461ES) and Recombinant DNase I (RNase-free) (Cat#10325ES).

Features

• Compliance with regulations: the products are fully validated according to the requirements of Chp, USP, ICH, etc., and their performance is in line with Chinese and foreign regulations and standards;
• Guarantee of quality: the raw materials of the kits are all self-developed, and the qPCR Mix and other enzyme products are produced in an ultra-clean enzyme factory;
• High sensitivity: the lower limit of quantification (LLOQ) was 5 copies/uL;
• High precision: high intra-batch reproducibility CV <10%, small inter-batch variation i.e. intermediate precision CV <15%;
• Strong specificity: specific detection of Lentivirus RNA without interference from other exogenous genomic DNA;
• Strong anti-interference: the introduction of internal quality control (IC) can exclude sample interference, abnormal reaction preparation and other factors, effectively avoiding false negatives.

Application

• Residual Lentivirus RNA test in biological products.

Specifications

Product Performance

Components

*IC：Internal control.

Storage

This product should be stored at -25~-15℃ for 1 years.

The 41333-A should be stored protected from light.

Figures

•  Limit of quantification

Lentivirus RNA at concentrations of 10 copies/uL, 5 copies/uL and 2 copies/uL were detected, with 10 replicates for each concentration, respectively. The results showed that the CV of Lentivirus RNA was <20% at concentrations of 5 copies/μL and above. Therefore, the limit of quantification in the Lentivirus RNA detection kits was 5 copies/μL for Lentivirus RNA.

 Table 1. Limit of quantification (LOQ) assay results for Lentivirus RNA

Figure 1. 5 copies/μL Lentivirus RNA qPCR assay results

• Limit of detection

The detection of Lentivirus RNA at various concentration points under the quantitative limit, combined with the blank limit requirements, yields the detection limit of the reagent kit. The results show that at concentrations of 0.5 copies/μL and above, in 20 repeated wells, ≥19 wells are detected (i.e., the detection rate is ≥95%). Therefore, the detection limit of the Lentivirus Physical Titer qPCR Detection Kit can reach 0.5 copies/μL.

Table 2. Limit of detection (LOD) assay results for Lentivirus RNA

Documents:

Safety Data Sheet

41333_MSDS_HB250310.pdf

Manuals

41333_Manual_Ver.EN20250310.pdf