Descripción
The SuperMethyl™ Max Bisulfite Conversion Kit from Ellis Bio offers the exceptionally mild yet powerful bisulfite conversion for DNA methylation analysis. Featuring a newly engineered ultra-mild bisulfite conversion formulation and a magnetic bead-based DNA purification method, this kit simplifies the methyl conversion workflow and achieves efficient conversion with minimal DNA degradation. Complete bisulfite conversion and DNA purification can be accomplished in 3 hours or less, preserving DNA integrity even with ultra-low input samples, while consistently achieving 99.8% conversion efficiency. Its broad applicability makes it a valuable tool for both research and translational settings.
Designed for compatibility and consistent performance in sensitive and/or low-input DNA samples, the SuperMethyl™ Max Bisulfite Conversion Kit delivers reliable, reproducible results, making it the preferred choice for precision-focused researchers.
Features
- Ultra-Mild Conversion Chemistry:Delivers excellent DNA library yield and larger library insert sizes, preserving sample integrity.
- Low-Input Friendly:Efficiently processes as little as 100 pg of DNA, ideal for limited or precious samples.
- Exceptional Conversion Efficiency:Achieves 99.8% C-to-T conversion, ensuring unmatched accuracy in methylation analysis.
- Uniform GC Coverage:Provides more even GC representation, enhancing coverage across promoters and GC-rich regions.
- Simplified, Rapid Workflow:Up to 3× faster with 5× less hands-on time than enzyme-based methods.
- Convenient Bead-Based Purification Format:Streamlined purification for improved yield and ease of use. Automation liquid handler friendly.
Component
|
Component |
Volume/Ǫuantity |
Storage |
|
Kit Capacity |
24 Tests |
Please see storage recommendations for individual components |
|
Conversion Reagent ◉ |
2 mL |
4 °C (recommended) |
|
Preparation Reagent ◉ |
120 µL |
Room temperature |
|
Enhancer A ◉ |
120 µL |
Room temperature |
|
Enhancer B ◉ |
240 µL |
4 °C (recommended) |
|
Binding Buffer |
22 mL |
Room temperature |
|
Wash Buffer (concentrate)* |
7 mL* |
Room temperature |
|
Desulphonation Buffer |
6 mL |
Room temperature |
|
Elution Buffer |
1 mL |
Room temperature |
|
Purification Beads ◉ |
400 µL |
Room temperature |
|
* Wash Buffer requires the addition of 28 mL 100% ethanol (EtOH) before first use. |
||
Shipping Conditions
No Cold Chain Required; Shipped at Room Temperature
Precautions / Handling Instructions
Upon receipt of the kit, store the Conversion Reagent and Enhancer B at 4°C.
Figures
1.
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
Yeasen se dedica a la ciencia ética y cree que nuestra investigación debe abordar cuestiones críticas al tiempo que garantiza la seguridad y los estándares éticos.