Descripción
The RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) is a kit capable of performing isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve efficient, rapid, and highly specific detection reactions. It also supports room-temperature reaction system preparation. The buffers and enzyme components in the kit do not contain glycerol and can be lyophilized using the provided lyophilization protectant.
Features
- Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
|
Components No. |
Name |
16730ES60 |
16730ES80 |
|
16730-A |
4×Lyo LAMP Buffer |
600 µL |
6 mL |
|
16730-B |
Bst Plus DNA Polymerase(Glycerol Free) |
100 µL |
1 mL |
|
16730-C |
RT Enzyme mix(Glycerol Free) |
50 µL |
500 µL |
|
16730-D |
25×Fluorescent Dye |
100 µL |
1 mL |
|
16730-E |
Lyophilization stabilizer |
800 µL |
8 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Application
Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow.
2. Consistent performance between lyophilized and liquid formats.
Figure 2. Comparable Detection Performance of Liquid and Lyophilized Formats.
Both liquid and lyophilized versions of the RT-LAMP assay exhibit consistent sensitivity and specificity in detecting DNA and RNA targets, with similar amplification curves and reaction times.
Documents:
Safety Data Sheet
Manuals
Pago y seguridad
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Consulta
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
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