Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
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Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA). Product Information Catalog No. 14415ES72 / 14415ES80 / 14415ES90 / 14415ES97 Size 2,500 U / 5,000 U / 50,000 U / 250,000 U Unit Definition One unit (U) is defined as the amount of enzyme...

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Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES

YeasenSku: 14415ES72
Size: 2,500 U
Precio:
$715.00
Existencias:
En stock

Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA).

Product Information

Catalog No.

14415ES72 / 14415ES80 / 14415ES90 / 14415ES97

Size

2,500 U / 5,000 U / 50,000 U / 250,000 U

Unit Definition

One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material within 30 min at 37°C.

Storage Buffer

25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.

Heat Inactivation

75°C for 20 min.

Component Information

Product Name

14415ES72
(2,500 U)

14415ES80
(5,000 U)

14415ES90
(50,000 U)

14415ES97
(250,000 U)

Bsu DNA Polymerase (Large Fragment, 50 U/μL)

50 μL

100 μL

1 mL

5 × 1 mL

Feature

  • Due to the lack of 3′-to-5′ exonuclease activity, Bsu DNA polymerase (Large Fragment) cannot remove unpaired 3′ overhangs and is therefore not suitable for generating blunt ends.
  • At 25°C, Bsu DNA polymerase (Large Fragment) retains 50% activity, which is twice that of Klenow Fragment (3′-to-5′ exo-) at the same temperature.
  • Storage buffer: 25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.
  • Heat inactivation: 75°C for 20 min.

Storage

Store at -25°C to -15°C. The shelf life is 2 years.

Documents:

Safety Data Sheet

14415_MSDS_HB260819

Manuals:

14415_Manual_HB20260818

Storage

Store at -25°C to -15°C. The shelf life is 2 years.

FAQ

Citations

Documents

Applications

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Preguntas frecuentes

El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.

Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.

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