Descripción
Hieff™ Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp. DNA Polymerase I and has been genetically engineered to eliminate its 5’→3’ exonuclease activity. This product exhibits strong 5’→3’ DNA polymerase activity, strand-displacement activity, and tolerance to dUTP, making it particularly suitable for contamination-controlled isothermal amplification reactions such as LAMP and CPA. Compared to Bst Plus DNA Polymerase, Hieff™ Super Bst DNA Polymerase demonstrates enhanced thermostability, which improves amplification performance. The enzyme is well-suited for low-template-concentration assays and high-temperature LAMP reactions (e.g., at 70°C), effectively enhancing detection specificity and shortening time-to-result. Its thermostable nature also enables the development of hot-start Bst formulations for further improvement in assay specificity.
Specifications
|
Cat NO. |
14411ES03 / 14411ES05 / 14411ES08 |
|
Size |
6,000 U / 24,000 U / 120,000 U |
Components
|
Components No. |
Name |
14411ES03 (6000 U) |
14411ES05 (24000 U) |
14411ES08 (120000 U) |
|
14411-A |
Hieff™ Super Bst DNA Polymerase(120 U/μL) |
50 μL |
200 μL |
1 mL |
|
14411-B |
10× Hieff™ Super Bst DNA Polymerase Buffer |
1 mL |
1 mL |
3 × 1 mL |
|
14411-C |
100 mM MgSO4 |
1 mL |
1 mL |
3 × 1 mL |
Applications
This product is suitable for various isothermal amplification methods, including LAMP, CPA, and RCA. It supports LAMP reactions at 70°C or, when combined with a thermostable reverse transcriptase, enables RT-LAMP at 70°C.
Unit Definition
One unit (U) is defined as the amount of enzyme that incorporates 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 65°C.
Enzyme Storage Buffer Composition
10 mM Tris-HCl, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol, pH 7.5 @ 25°C.
Storage
Store at –25 to –15°C for 2 years. Avoid repeated freeze-thaw cycles.
Notes
1. Keep the enzyme on ice or in an ice bath during use, and return it immediately to –20°C after use.
2. This product is for research use only.
3. For your safety and health, wear a lab coat and disposable gloves during handling.
LAMP Experimental Example
1. Reaction Setup
|
Component |
Volume (μL) |
Final Concentration |
|
10× Hieff™ Super Bst DNA Polymerase Buffer |
2.5 |
1× |
|
100 mM MgSO₄ |
0.75 |
3 mM + 2 mM in buffer = 5 mM |
|
dNTP Mix (25 mM each) |
1.4 |
1.4 mM each |
|
dUTP (25 mM) (optional) |
1.4 |
1.4 mM |
|
UDGase (1 U/μL) (optional) |
1 |
0.04 U |
|
Template DNA |
10 ng–1 μg |
— |
|
10× Primers |
2.5 |
— |
|
Hieff™ Super Bst DNA Polymerase (120 U/μL) |
0.34–1* |
— |
|
ddH₂O |
to 25 |
— |
[Note]: The amount of Hieff™ Super Bst DNA Polymerase may be optimized according to specific experimental requirements.
10× Buffer composition: 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgSO₄, 1% Tween-20, pH 8.8 @ 25°C.
Mg²⁺ concentration can be adjusted between 4–10 mM depending on the assay.
10× Primers: 16 µM FIP/BIP, 2 µM F3/B3, 4 µM Loop F/B each.
Our dNTP (Cat#10124), dUTP (Cat#10128), and UDGase (Cat#10303) are compatible with this product.
2. Reaction Conditions
|
Temperature |
Time |
Purpose |
|
25–37°C |
5–10 min |
Degradation of uracil-containing templates (optional) |
|
65–70°C |
30–60 min |
Amplification |
|
85°C |
5 min |
Enzyme inactivation |
Documents:
Safety Data Sheet
Manuals
Pago y seguridad
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Consulta
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Preguntas frecuentes
El producto es solo para fines de investigación y no está destinado a uso terapéutico o diagnóstico en humanos o animales. Los productos y el contenido están protegidos por patentes, marcas comerciales y derechos de autor propiedad de Yeasen Biotechnology. Los símbolos de marca comercial indican el país de origen, no necesariamente el registro en todas las regiones.
Algunas aplicaciones pueden requerir derechos de propiedad intelectual adicionales de terceros.
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