The CpG cytosines on the sequence of Hieff™ Methylated PUC19 DNA are fully methylated, which can be used as a known "positive" standard to evaluate the efficiency and accuracy of the experimental workflow. After sequencing, theoretically the C on CpG will not be converted. By calculating how many C have been converted to T, the over-conversion efficiency of bisulfite in this experiment can be evaluated, ensuring the reliability of subsequent analysis. The full length of the PUC19 DNA sequence is 2686 bp.
Specifications
|
Product Name |
Catalog No. |
Size |
|
Hieff™ Methylated PUC19 DNA |
12233ES50 |
50 ng |
Storage
The product should be stored at -25°C to -15°C for 2 years.
Notes
1. As a "spike-in" (positive control), it is recommended to spike into real samples at a ratio of 0.1%–1% and proceed with the entire experimental workflow together.
2. For your safety and health, please operate with lab coats and disposable gloves.
3. This product is for research use only.
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