Beschreibung
Hieff NGSTM dsDNA Methyl Library Prep Kit for IlluminaTM is a double-stranded DNA methylation library kit. This kit can construct a methylation library for Illumina platform sequencing by connecting methylated adapters to DNA that has been treated with bisulfite or enzymatic conversion, and then amplifying it in one step. All components of the kit have passed quality control and functional verification to ensure stable output of the library, and support a variety of DNA samples, including gDNA, cfDNA, and FFPE. This kit is compatible with 10 ng-1000 ng DNA and is compatible with mainstream conversion schemes on the market.
Features
- Easy operation: The double-stranded methylation library workflow is simpler than single-stranded library preparation, requiring one less adapter ligation and purification step.
- Simplified analysis: Double-stranded methylation library data analysis does not require read trimming, resulting in more usable data.
- High hybrid capture mapping: Hybrid capture achieves high mapping rates without the need for special blocking reagents.
Components
|
Components No. |
Name |
12214ES08 |
12214ES24 |
12214ES96 |
|
|
12214-A |
|
Endprep Buffer |
56 μL |
168 μL |
672 μL |
|
12214-B |
|
Endprep Enzyme |
24 μL |
72 μL |
288 μL |
|
12214-C |
|
Ligation Enhancer |
240 μL |
720 μL |
3×960 μL |
|
12214-D |
|
Rapid T4 DNA Ligase |
80 μL |
240 μL |
960 μL |
|
12214-E |
|
Methyl Stubby Adapter |
40 μL |
120 μL |
480 μL |
|
12214-F |
|
2 × CanaceTM Uracil + PCR Mix 2.0 |
200 μL |
600 μL |
2×1200 μL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year..
Application
Whole-genome bisulfite sequencing (WGBS); Methylated DNA immunoprecipitation sequencing (MeDIP-seq); Methylation-sensitive restriction enzyme sequencing; Cancer methylation biomarker research; Epigenetic regulation studies; DNA methylation analysis in development; Environmental impact on DNA methylation; Epigenetics research in plants and animals
Figures
1. Workflow
Figure 1. Workflow of double-stranded DNA methylation library preparation
2. Robust Library Yield
Figure 2. Comparison of library yields from different sample types.
Using HieffTM Mag DNA Methylation Bisulfite Kit(Cat#12223), gDNA, cfDNA, and FFPE samples with inputs of 10 ng, 100 ng, and 1 μg were prepared. Library yields outperformed those from Supplier A* across all input amounts, demonstrating consistent performance across different sample types.
3. Excellent Sequencing Data Performance
Figure 3. Performance comparison between dsDNA and ssDNA methylation library prep(Kit 12214: Double-stranded library prepKit; 12221: Single-stranded library prep kit; BS: Bisulfite conversion; EM: Enzymatic conversion)
Documents:
Safety Data Sheet
Manuals
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FAQ
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