In vitro mRNA synthesis produces mRNA for vaccines and gene therapy. A linearized DNA template with a promoter (e.g., T7 promoter: 5'-TAATACGACTCACTATAG-3' ) is transcribed by T7 RNA polymerase using nucleotides, buffer, and magnesium ions. A 5' cap is added via a capping enzyme or cap analog, and a poly-A tail is included using the template or poly-A polymerase for stability. DNase I removes residual DNA, and the mRNA is purified. Key enzymes—BspQ1, T7 RNA polymerase, Pyrophosphatase, Inorganic, Murine RNase inhibitor, capping enzyme, poly-A polymerase, and DNase I—ensure functional mRNA.
Yeasen
Cleascript ™ T7 -RNA -Polymerase (niedrige dsRNA, 250 u/μl) _ 10628es
VerkaufspreisAus $180.00
Yeasen
Cleascript ™ T7-RNA-Polymerase (GMP-Grad, niedrige dsRNA, 250 u/μl) _ 10629es
VerkaufspreisAus $198.00
Speichern $40.00
