Beschreibung
Tko RNA Helicase is derived from the TK0306 gene of the thermophilic archaeon Thermococcus kodakaraensis (strain KOD1) and belongs to the DEAD-box RNA helicase family. This enzyme is ATP-dependent and specifically unwinds single-stranded stem-loop RNA structures. It exhibits weak activity towards double-stranded RNA (dsRNA) and single-stranded DNA (ssDNA) substrates and does not act on dsRNA substrates with 5' or 3' overhangs. With an optimal temperature of 50°C, this enzyme serves as a useful tool for specifically unwinding RNA stem-loop structures under mild temperature conditions, making it suitable for applications such as RNA structural analysis and post-transcriptional regulation research.
Specifications
|
Cat.No. |
14619ES08 / 14619ES20 |
|
Size |
5 μg/ 20 μg |
|
Reaction Temperature |
50℃ |
|
Inactivation Conditions |
Incubate at 80°C for 5 minutes |
Components
|
Components No. |
Name |
14619ES08 |
14619ES20 |
|
14619-A |
Tko RNA Helicase (100 µg/mL) |
50 μL |
200 μL |
|
14619-B |
10×Tko Reaction Buffer |
1 mL |
1.5 mL |
Storage
This product should be stored at -25~-15℃ for 1 year.
Instructions
1. Prepare the following reaction mixture on ice
|
Component |
Volume(μL） |
|
10×Tko Reaction Buffer |
2 |
|
ssRNA (5–10 nM) |
1 |
|
Tko RNA Helicase (100 µg/mL) |
0.5–1 |
|
Murine RNase inhibitor(40 U/μL) |
0.1 |
|
DEPC H2O |
Up to 20 μL |
2. Incubate at 50°C for 30 min.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
3. The reaction buffer for this enzyme requires 1–2 mM ATP or dATP and 2 mM Mg²⁺. The 10×Tko Reaction Buffer already contains ATP and Mg²⁺.
4. For different substrates, the enzyme amount should be adjusted; gradient testing is recommended.
Documents
Zahlung und Sicherheit
Ihre Zahlungsinformationen werden sicher verarbeitet. Wir speichern weder Kreditkartendaten noch Zugriff auf Ihre Kreditkarteninformationen.
Sie können auch mögen
Anfrage
FAQ
Das Produkt ist nur für Forschungszwecke bestimmt und nicht für die therapeutische oder diagnostische Anwendung bei Menschen oder Tieren. Produkte und Inhalte sind durch Patente, Marken und Urheberrechte von
Für bestimmte Anwendungen sind möglicherweise zusätzliche geistige Eigentumsrechte Dritter erforderlich.