Hieff Clone™ Zero TOPO-TA/Blunt Cloning Kit is developed based on the principle of a next-generation topoisomerase that enables rapid and efficient ligation of DNA fragments. Coupled with a highly efficient blunt-ending factor, this kit supports fast cloning of both blunt-ended and sticky-ended PCR products.

Features

• Fast: Ligation completes in just 5 minutes.
• Highly Efficient: Virtually no self-ligation; near 100% positive clone rate—no blue-white screening needed.
• Broad Insert Range: Supports fragments from 150 bp to 5 kb.
• Simple Workflow: Just add your purified insert—no extra steps required.
• Versatile Compatibility: Enhanced with blunt-end binding factor for seamless TA cloning and blunt-end cloning.

Components

Storage

This product should be stored at room temperature for 1 year.

Application

Molecular Cloning; Topo Cloning; Rapid Cloning

Figures

1. Versatile Compatibility: TA cloning and blunt-end cloning.

 Figure 1. High cloning efficiency of the Hieff Clone™ Universal Zero TOPO TA/Blunt Cloning Kit.

With 10 ng of a 1 kb insert (either blunt- or sticky-ended), the kit yields abundant colonies and achieves 100% positive clone rate.

2. Stong Stability

(A).Accelerated Stability

 (B).Freeze-Thaw Stability 

Figure 2. Stability Assessment

(A).Accelerated Stability: The Hieff Clone® Universal Zero TOPO TA/Blunt Cloning Kit remains stable after 7 days at 37°C.

(B).Freeze-Thaw Stability: The kit maintains performance after 50 freeze-thaw cycles.

Documents:

Safety Data Sheet

10906_MSDS_HB251120_EN.PDF

Manuals

10906_Manual_Ver.EN20251120.pdf