Agarose -10208ES

YeasenSKU: 10208ES08

Size: 5 g
سعر:
سعر البيع$15.00
مخزون:
في الأوراق المالية

Agarose is a purified linear galactoglycan hydrophilic colloid derived from agar or agar-containing algae. It is a linear polymer composed of β-d-galactosyl (1-4) linked to 3, 6-dehydrated-L-galactosyl. As a gel reagent, it is commonly used for routine nucleic acid analysis by gel electrophoresis or blotting (such as Northern or Southern) and for protein applications, such as radiative immunodiffusion (RID) assay.

Applications

Gel electrophoresis

Components

Name

10208ES08

10208ES60

10208ES70

Agarose

5 g

100 g

500 g

Specifications

CAS#

9012-36-6

Appearance

White to off-white powder

Gel Strength (1.0%)

≥1200 (g/cm2)

Gel Point (1.5%)

36±1.5℃

Melting Point (1.5%)

88±1.5℃

EEO (-mr)

≤0.13

Sulfate (%)

≤0.15%

Moisture

≤10%

DNase

None Detected

RNase

None Detected

Protease

None Detected

Storage

The Agarose products should be stored at room temperature for 5 years.

FAQ

It is sterile and nuclease-free. Low melting point agarose is commonly used for plaque assays, so it can be used safely for cell culture. Other types of agarose are also sterile and nuclease-free.
The firmness is related to the gel strength. Agarose is extracted from agar, and there will always be some variation in strength levels; it is impossible for every batch to be identical. The gel strength specification for this agarose is $\ge 1200$, and as long as it is above this range, it is considered qualified.
The gelling time may have been too short, resulting in an uneven gel. For a 1% agarose gel, the minimum gelling time is 30 minutes.
The resolution for both high-resolution agarose and 3:1 agarose is around 2% (meaning if the fragment size is 30 bp, it can separate $30 \times 0.02$; for a 100 bp fragment, $100 \times 0.02$).
No, pulsed-field gel electrophoresis requires very low electroosmosis and very high gel strength.

Citations

Applications

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